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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)D5MTG8[108 - 179]
Description
Feature keyCompositional bias
Feature identifier
        10         20         30         40         50
MTTASNTIIT GTSTGKLPGE VPEALQVNGT TPSGKPRLFV CSTCTRAFAR
60 70 80 90 100
QEHLKRHERS HTKEKPFSCG VCSRKFSRRD LLLRHAQKLH AGCSDTAITR
110 120 130 140 150
LRKKSIRRSS TSSSIGSTPM NHNNSTNDDS VSSTIRSKKM VRGSISGASS
160 170 180 190 200
SMNNNSINNH ANNNSSFSTG SISKPRASSI SNLKNSNKIE TIKTILENKT
210 220 230 240 250
EDPIQNYNNN HNLNNMGGHN SISPSSSLSS NFVVENQFNM GQNDSKNTYN
260 270 280 290 300
NFNNSNGNGN GNGNGNGNGN NHVATNNSSN NNLQQFNEDV LTDLPFLRNH
310 320 330 340 350
LPMNRRASFS AMSGNNYATS FNQPYASETV EFATPQMMPM DDAENWLNNL
360 370 380 390 400
DEIPSLDFIN PNFQQKQHDQ NNQQQQQQQQ QQKYHLQQQQ QFQNQNQQTQ
410 420 430 440 450
LKNESPAEAI SGYSFYDVPS KNVESLFSHS NRMPNSNNNG NNNSNINNSS
460 470 480 490 500
NLTQNMLNNH LNSLLLSPLL HDSPDIDSSP SSQAQVWSNF NNNNNGRQHS
510 520 530 540 550
KLANNSNGNK NNNDNNSSKS KVHNFNNDIN ELELLTDLNA SRDYNLPAGY
560 570 580 590 600
SFYGSTDIQS NLNNNSSCSS SNATISPILL DNAYGNSSTI SNQPPNNLNS
610 620 630 640 650
NSSFNSPFSR PNTTSQQQQQ TLPQQQQHQH HQHHQQHQSH YNDQQQQLNS
660 670 680 690 700
EVKNIPNNIS NNFEKYSTNL LFTNLIRNCI HFSLSKYPFI GLQNPTIPDN
710 720 730 740 750
DKLNFYTKVF EEKFLLHHPF IHKSYLNEFS LIKTTLKNIN TNKLDDLANS
760 770 780 790 800
NGNINSSGTS DYIKVSLIWF PLLVATIGAV YWNRKADAAN LYEASRRSIP
810 820 830 840 850
VYLDFRKRQQ PPMQNQQNGE TPQLNDNSSP LWLIQSLTLS IIYGLFADND
860 870 880 890 900
ICLNVIIRQV NALNSLLKSS GLTMISWNYD SNKNIDDKYF ENYIQYESTV
910 920 930 940 950
RTIHTIFHIS AMLSSLYNIV PSLKVDELFI DLPVNSIVWN TNNTNEFKQV
960 970 980 990 1000
FMTFIHSKPE KFSKILGDLL EFPFNSFHSS SISPGSSTHS SSSYHSTRSL
1010 1020 1030 1040 1050
SVKNFLADNQ ISEFGLNCLL NGLHQYNYFQ QLAESNDANN NNNNNNDNNN
1060 1070 1080 1090 1100
TGVHNNSNID ANLLDKLSNS QKYSSVSNNH TNQFSNISNE VKSIRVDTRI
1110 1120 1130 1140 1150
QDIINSWDNM ISGSSIYDQN SELVNDCKIL NHYLSIKSSG IISLNRVKES
1160 1170 1180 1190 1200
VWLKSWNDMC ELYYNTYKFK EADILRDNTL SDNLRDVVGH SVDILKLVLV
1210 1220 1230 1240 1250
FFKKIDKSSS ISSLSSAVGG SKIGEAVNSL TLNEDQLKEI TDENLGELDF
1260 1270 1280 1290 1300
ENFSKHLSID LQILFDVFLV LVKFLITFER NSKIKMRYNN LNSNINFVHH
1310 1320 1330 1340 1350
FELQSNILPF NYNTNSAING PIPGLSENLN SISLNGNTNK GGHIGNEEDG
1360 1370 1380 1390 1400
DEAARSKQND LEFFKLYKLI FKLFLNLEFF LKVNYGYHDF ESEFANLTIS
1410 1420 1430 1440 1450
SIVNKYKNKD INIGIDSNVN KFVDGNNYDK LHGSKLHNNT SDEDLSPRDR
1460 1470 1480 1490 1500
DLIINELINF KLPFKLLKIG EFLFSFLYDK NIKFVNFKNL SSGLFHLRIF

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