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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)G5EFD2[630 - 630]
Description
Feature keyMutagenesis
Feature identifier
        10         20         30         40         50
MMQTQVASRA GYSNLPQFGA GIAQDIKTQA INNLKECLKL TTINRFLTSS
60 70 80 90 100
YEEDAKSVER KIFSAVYQMT KIGLIDREKR EINAIWFTFV GLSAQNIRHL
110 120 130 140 150
EICSITDFNA LFSITNQELR SLADRGRLDV ETKRKLLQST VILQNHWNAY
160 170 180 190 200
HSRTSSGSTD EPSGQSTPAI VTPSPKFNVP SLSVTSAKMI QSSSMGFATT
210 220 230 240 250
PKSPKTSSRL VHAIPHKWHR STKFRFSGDA VCHFCQRPLG FGFLNAWEKC
260 270 280 290 300
RSCKWKVHTQ CKGRVGDSCG LTPDHLRFLF DKLIQENNGG MWKDPQSVPG
310 320 330 340 350
SRSMNEPAFQ FPDTAIDSSS STNSSAPSTP ALPAGISGNV SSLTAPYRSE
360 370 380 390 400
RKFLFPDTEN YSVHNRLPIL VISEGDHPTT TEIQQETENH NKSAAASMSG
410 420 430 440 450
NIESEGTIVA NHEDSTGSQE VDSEAAPSQE AVDKFNKRAD GGFTWERHAW
460 470 480 490 500
NMSTIRGPNA QASWNEVTIQ FETIEFDKQA PIIGRGRFGK VLRGFHYGDV
510 520 530 540 550
AVKVYTMEHI SDASKKAEEF KLEVSAYKNT RHDNIALFLG YFMSDGQYGM
560 570 580 590 600
VMSLSKGSQS LYTLLHVVRE KLDLATTRKI AQQICQAVSY LHTKKILHKD
610 620 630 640 650
LRSKNILLES KNKVVITDFG ILSMKRLAHP KQKSGYLTSK FWTNYIAPEL
660 670 680 690 700
AMAMRTEYDE YECDDFPFSE NSDVYAFGCV WFEMLTGALP YAGELPHQIL
710 720 730 740 750
FAKTQGIRPV LPNVKCTQEL KELLVSCWNT APQDRPTLTD INLKLTALPK
760 770
KPRVNRSPSF PVMMKSYEST F
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