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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)P0C0K7[1011 - 1013]
Description
Feature keyMotif
Feature identifier
        10         20         30         40         50
MASENTAGSG SRVAGMVYSL WLLVLGPSVL ALEEVLLDTT GETSEIGWLT
60 70 80 90 100
YPPGGWDEVS VLDDQRRLTR TFEACHVAGL PPGSGQDNWL QTHFVERRGA
110 120 130 140 150
QRAHIRLHFS VRACSSLGVS GGTCRETFTL YYRQADEPDS PDSISAWHLK
160 170 180 190 200
RWTKVDTIAA DESFPASSSS SSWAVGPHRT DQRVGLQLNV KERSFGPLTQ
210 220 230 240 250
RGFYVAFQDT GACLALVAVK LFSYTCPSVL RAFASFPETQ ASGAGGASLV
260 270 280 290 300
AAVGTCVAHA EPEEDGVGGQ AGGSPPRLHC NGEGRWMVAV GGCRCQPGHQ
310 320 330 340 350
PARGDKLCQA CPEGSYKALP GNVPCSPCPA RSHSPDPAAP VCPCLQGFYR
360 370 380 390 400
ASSDPPEAPC TGPPSAPREL WFEVQGSALM LHWRLPQELG GRGDLLFNVV
410 420 430 440 450
CKECGGHKEP SSGGMCRRCR DEVHFDPRQR GLTESRVLVG GLRAHVPYIL
460 470 480 490 500
EVQAVNGVSE LSPDPPQAAA INVSTSHEVP SAVPVMHQVS RAANSITVSW
510 520 530 540 550
PQPEQTNGNI LDYQLRYYDQ AEDESHSFTM TSETNTATVT RLSPGHIYGF
560 570 580 590 600
QVRARTAAGH GPYGGKVYFQ TLPQGELSSQ LPEKLSLVIG SILGALAFLL
610 620 630 640 650
LAAITVLAVI FQRKRRGTGY TEQLQQYSSP GLGVKYYIDP STYDDPCQAI
660 670 680 690 700
RELAREVDPT YIKIEEVIGA GSFGEVRRGR LQPRGRREQA VAIQALWAGG
710 720 730 740 750
AESLKMTFLG RAALLGQFQH PNILRLEGVV TRSRPVMVLT ELMELGPLDS
760 770 780 790 800
FLRQREGQFS SLQLVAMQRG VAAAMQYLSS FAFVHRALSA RSVLVNSHLV
810 820 830 840 850
CKVARLGHSP QGSSSLLRWA APEVITHGKY TTSSDVWSFG ILMWEVMSYG
860 870 880 890 900
ERPYWDMSDQ EVLNAIEQEF RLPPPPGCPT GLHLLMLDTW QKDRARRPHF
910 920 930 940 950
DQLVAAFDKM IRKPDTLQAE GSSGDRPSQA LLNPVALDFP CLDSPQAWLS
960 970 980 990 1000
AIGLECYQDN FSKFGLSTFS DVAQLSLEDL PGLGITLAGH QKKLLHNIQL
1010
LQQHLRQPGS VEV
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Main funding by: National Institutes of Health

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