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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)P28481-5
Description
Feature key
Feature identifier
        10         20         30         40         50
MIRLGAPQSL VLLTLLIAAV LRCQGQDAQE AGSCLQNGQR YKDKDVWKPS
60 70 80 90 100
SCRICVCDTG NVLCDDIICE DPDCLNPEIP FGECCPICPA DLATASGKLG
110 120 130 140 150
PKGQKGEPGD IRDGPAGEQG PRGDRGDKGE KNFAAQMAGG YDEKAGGAQM
160 170 180 190 200
GVMQGPMGPM GPRGPPGPAG APGPQGFQGN PGEPGEPGVS GPMGPRGPPG
210 220 230 240 250
PAGKPGDDGE AGKPGKSGER GLPGPQGARG FPGTPGLPGV KGHRGYPGLD
260 270 280 290 300
GAKGEAGAPG VKGESGSPGE NGSPGPMGPR GLPGERGRTG PAGAAGARGN
310 320 330 340 350
DGQPGPAGPP GPVGPAGGPG FPGAPGAKGE AGPTGARGPE GAQGSRGEPG
360 370 380 390 400
NPGSPGPAGA SGNPGTDGIP GAKGSAGAPG IAGAPGFPGP RGPPGPQGAT
410 420 430 440 450
GPLGPKGQAG EPGIAGFKGD QGPKGETGPA GPQGAPGPAG EEGKRGARGE
460 470 480 490 500
PGGAGPIGPP GERGAPGNRG FPGQDGLAGP KGAPGERGPS GLTGPKGANG
510 520 530 540 550
DPGRPGEPGL PGARGLTGRP GDAGPQGKVG PSGAPGEDGR PGPPGPQGAR
560 570 580 590 600
GQPGVMGFPG PKGANGEPGK AGEKGLAGAP GLRGLPGKDG ETGAAGPPGP
610 620 630 640 650
SGPAGERGEQ GAPGPSGFQG LPGPPGPPGE GGKQGDQGIP GEAGAPGLVG
660 670 680 690 700
PRGERGFPGE RGSPGAQGLQ GPRGLPGTPG TDGPKGAAGP DGPPGAQGPP
710 720 730 740 750
GLQGMPGERG AAGIAGPKGD RGDVGEKGPE GAPGKDGGRG LTGPIGPPGP
760 770 780 790 800
AGANGEKGEV GPPGPSGSTG ARGAPGERGE TGPPGPAGFA GPPGADGQPG
810 820 830 840 850
AKGDQGEAGQ KGDAGAPGPQ GPSGAPGPQG PTGVTGPKGA RGAQGPPGAT
860 870 880 890 900
GFPGAAGRVG PPGANGNPGP AGPPGPAGKD GPKGVRGDSG PPGRAGDPGL
910 920 930 940 950
QGPAGAPGEK GEPGDDGPSG LDGPPGPQGL AGQRGIVGLP GQRGERGFPG
960 970 980 990 1000
LPGPSGEPGK QGAPGASGDR GPPGPVGPPG LTGPAGEPGR EGSPGADGPP
1010 1020 1030 1040 1050
GRDGAAGVKG DRGETGALGA PGAPGPPGSP GPAGPTGKQG DRGEAGAQGP
1060 1070 1080 1090 1100
MGPSGPAGAR GIAGPQGPRG DKGESGEQGE RGLKGHRGFT GLQGLPGPPG
1110 1120 1130 1140 1150
PSGDQGASGP AGPSGPRGPP GPVGPSGKDG SNGIPGPIGP PGPRGRSGET
1160 1170 1180 1190 1200
GPVGPPGSPG PPGPPGPPGP GIDMSAFAGL GQREKGPDPM QYMRADEADS
1210 1220 1230 1240 1250
TLRQHDVEVD ATLKSLNNQI ESIRSPDGSR KNPARTCQDL KLCHPEWKSG
1260 1270 1280 1290 1300
DYWIDPNQGC TLDAMKVFCN METGETCVYP NPATVPRKNW WSSKSKEKKH
1310 1320 1330 1340 1350
IWFGETMNGG FHFSYGDGNL APNTANVQMT FLRLLSTEGS QNITYHCKNS
1360 1370 1380 1390 1400
IAYLDEAAGN LKKALLIQGS NDVEMRAEGN SRFTYTALKD GCTKHTGKWG
1410 1420 1430 1440
KTVIEYRSQK TSRLPIIDIA PMDIGGAEQE FGVDIGPVCF L
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