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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)P78820[1362 - 1362]
Description
Feature keySequence conflict
Feature identifier
        10         20         30         40         50
MRQSVTSSSS CSKLYAIKAT ISLPRLFYRR LRTMAPRVAS HFLGGNSLDK
60 70 80 90 100
APAGKVKDYI ASHGGHTVIT SILIANNGIA AVKEIRSIRK WAYETFNNER
110 120 130 140 150
AIKFTVMATP DDLKVNADYI RMADQYVEVP GGSNNNNYAN VELIVDIAER
160 170 180 190 200
MNVHAVWAGW GHASENPKLP EMLSASSKKI VFIGPPGSAM RSLGDKISST
210 220 230 240 250
IVAQSARVPC MSWSGNELDQ VRIDEETNIV TVDDDVYQKA CIRSAEEGIA
260 270 280 290 300
VAEKIGYPVM IKASEGGGGK GIRQVTSTEK FAQAFQQVLD ELPGSPVFVM
310 320 330 340 350
KLAGQARHLE VQILADQYGN NISLFGRDCS VQRRHQKIIE EAPVTIAPAA
360 370 380 390 400
TFHEMERAAV RLGELVGYAS AGTIEYLYEP ENDRFYFLEL NPRLQVEHPT
410 420 430 440 450
TEMVSGVNLP AAQLQVAMGL PLSRIPHIRE LYGLPRDGDS EIDFFFQNPE
460 470 480 490 500
SFKVQKVPTP KGHCVACRIT SEDPGEGFKP SSGMIKDLNF RSSSNVWGYF
510 520 530 540 550
SVGTAGGIHE FADSQFGHIF SFAESRESSR KSMVVALKEL SIRGDFRTTV
560 570 580 590 600
EYLVRLLETK EFSENEFTTG WLDRLIAQKV TSARPDKMLA VVCGALVRAH
610 620 630 640 650
ATADTQYRAF KSYLERGQVP SREFLKNVYD IEFIYDNTRY RFTASRSSPG
660 670 680 690 700
SYHLFLNGSR CTAGVRSLTD GGLLVLLNGH SYTVYYRDEV TGTRISIDNL
710 720 730 740 750
SCMLEQENDP TQLRTPSPGK LVRFLVETGE HIKAGEAYAE VEVMKMIMPL
760 770 780 790 800
VATEDGVVQL IKQPGASLDA GDILGILTLD DPSRVTHALP FDGQLPNWGE
810 820 830 840 850
PQIAGNKPCQ RYHALLCILL DILKGYDNQI ILNSTYNEFV EVLRNHELPY
860 870 880 890 900
SEWSAHYSAL VNRISPVLDK LFVSIIEKAR SRKAEFPAKQ LEVAIQTYCD
910 920 930 940 950
GQNLATTQQL KVQIAPLLKI ISDYKDGLKV HEYNVIKGLL EEYYNVEKLF
960 970 980 990 1000
SGINKREEDV ILRLRDENKD DVDKVIALAL SHSRIGSKNN LLITILDLMK
1010 1020 1030 1040 1050
SEPSTFVSLY FNDILRKLTD LDSRVTSKVS LKARELLITC AMPSLNERFS
1060 1070 1080 1090 1100
QMEHILKSSV VESHYGDAKF SHRTPSLDIL KELIDSKYTV FDVLPAFFCH
1110 1120 1130 1140 1150
TDPWVSLAAL EVYVRRAYRA YSVLEINYHT EAGTPYVLTW RFQLHSSGAP
1160 1170 1180 1190 1200
GLGANSTNGS NFPASTTPSY ENSNRRLQSV SDLSWYVNKT DSEPFRFGTM
1210 1220 1230 1240 1250
IAAETFDELE NNLALAIDRL PLSRNYFNAG LTLDGNSSSA NDNTQELTNV
1260 1270 1280 1290 1300
VNVALTSTGD LDDSAIVSKL NQILSDFRDD LLEHNVRRVT IVGGRINKSA
1310 1320 1330 1340 1350
YPSYYTYRVS AEQKDGNLVH YNEDERIRHI EPALAFQLEL GRLSNFNIEP
1360 1370 1380 1390 1400
VFTDNHNIHV YRATAKNMDT DKRFFTRALV RPGRLRDEIP TAEYLISETH
1410 1420 1430 1440 1450
RLINDILDAL EVIGHEQTDL NHIFINFTPA FGLAPKQVEA ALGGFLERFG
1460 1470 1480 1490 1500
RRLWRLRVTA AEIRIICTDP STNTLFPLRV IISNVSGFVV NVEIYAEVKT
1510 1520 1530 1540 1550
ENNSWIFKSI GQPGSMHLRP ISTPYPTKEW LQPRRYKAQL MGTTFVYDFP
1560 1570 1580 1590 1600
ELFRRAFTDS WKKVPNGRSK VTIPQNMFEC KELVADEHGV LQEVNREPGT
1610 1620 1630 1640 1650
NSCGMVAWCI TVKTPEYPNG RKIIVVANDI TFQIGSFGPQ EDEYFYKVTQ
1660 1670 1680 1690 1700
LARQRGIPRI YLAANSGARI GVADEIVPLF NIAWVDPDSP EKGFDYIYLT
1710 1720 1730 1740 1750
PEAYERLQKE NPNILTTEEV VTETGELRHK ITTIIGSSEG LGVECLRGSG
1760 1770 1780 1790 1800
LIAGVTSRAY NDIFTCTLVT CRAVGIGAYL VRLGQRAVQI EGQPIILTGA
1810 1820 1830 1840 1850
PALNKVLGRE VYTSNLQLGG TQVMHRNGIS HLTSQDDFDG ISKIVNWISY
1860 1870 1880 1890 1900
IPDKRNNPVP ISPSSDTWDR DVEFYPSQNG YDPRWLIAGK EDEDSFLYGL
1910 1920 1930 1940 1950
FDKGSFQETL NGWAKTVVVG RARMGGIPTG VIAVETRTIE NTVPADPANP
1960 1970 1980 1990 2000
DSTEQVLMEA GQVWYPNSAF KTAQAINDFN HGEQLPLFIL ANWRGFSGGQ
2010 2020 2030 2040 2050
RDMFNEVLKY GSYIVDALAS YKQPVFVYIP PFSELRGGSW VVVDPTINED
2060 2070 2080 2090 2100
QMEMYADEES RAGVLEPEGM VSIKFRREKL LSLMRRCDHK YASLCNELKR
2110 2120 2130 2140 2150
DDLSADDLST IKVKLMEREQ KLMPIYQQIS IHFADLHDRV GRMVAKKVVR
2160 2170 2180 2190 2200
KPLKWTEARR FFYWRLRRRL NEHYALQKIT QLIPSLTIRE SREYLQKWYE
2210 2220 2230 2240 2250
EWCGKQDWDE SDKSVVCWIE EHNDDLSKRT QELKSTYYSE RLSKLLRSDR
2260 2270 2280
KGMIDSLAQV LTELDENEKK ELAGKLASVN
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Main funding by: National Institutes of Health

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