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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q15155[659 - 659]
Description
Feature keySequence conflict
Feature identifier
        10         20         30         40         50
MLVGQGAGPL GPAVVTAAVV LLLSGVGPAH GSEDIVVGCG GFVKSDVEIN
60 70 80 90 100
YSLIEIKLYT KHGTLKYQTD CAPNNGYFMI PLYDKGDFIL KIEPPLGWSF
110 120 130 140 150
EPTTVELHVD GVSDICTKGG DINFVFTGFS VNGKVLSKGQ PLGPAGVQVS
160 170 180 190 200
LRNTGTEAKI QSTVTQPGGK FAFFKVLPGD YEILATHPTW ALKEASTTVR
210 220 230 240 250
VTNSNANAAS PLIVAGYNVS GSVRSDGEPM KGVKFLLFSS LVTKEDVLGC
260 270 280 290 300
NVSPVPGFQP QDESLVYLCY TVSREDGSFS FYSLPSGGYT VIPFYRGERI
310 320 330 340 350
TFDVAPSRLD FTVEHDSLKI EPVFHVMGFS VTGRVLNGPE GDGVPEAVVT
360 370 380 390 400
LNNQIKVKTK ADGSFRLENI TTGTYTIHAQ KEHLYFETVT IKIAPNTPQL
410 420 430 440 450
ADIIATGFSV CGQISIIRFP DTVKQMNKYK VVLSSQDKDK SLVTVETDAH
460 470 480 490 500
GSFCFKAKPG TYKVQVMVPE AETRAGLTLK PQTFPLTVTN RPMMDVAFVQ
510 520 530 540 550
FLASVSGKVS CLDTCGDLLV TLQSLSRQGE KRSLQLSGKV NAMTFTFDNV
560 570 580 590 600
LPGKYKISIM HEDWCWKNKS LEVEVLEDDM SAVEFRQTGY MLRCSLSHAI
610 620 630 640 650
TLEFYQDGNG RENVGIYNLS KGVNRFCLSK PGVYKVTPRS CHRFEQAFYT
660 670 680 690 700
YDTSSPSILT LTAIRHHVLG TITTDKMMDV TVTIKSSIDS EPALVLGPLK
710 720 730 740 750
SVQELRREQQ LAEIEARRQE REKNGNEEGE ERMTKPPVQE MVDELQGPFS
760 770 780 790 800
YDFSYWARSG EKITVTPSSK ELLFYPPSME AVVSGESCPG KLIEIHGKAG
810 820 830 840 850
LFLEGQIHPE LEGVEIVISE KGASSPLITV FTDDKGAYSV GPLHSDLEYT
860 870 880 890 900
VTSQKEGYVL TAVEGTIGDF KAYALAGVSF EIKAEDDQPL PGVLLSLSGG
910 920 930 940 950
LFRSNLLTQD NGILTFSNLS PGQYYFKPMM KEFRFEPSSQ MIEVQEGQNL
960 970 980 990 1000
KITITGYRTA YSCYGTVSSL NGEPEQGVAM EAVGQNDCSI YGEDTVTDEE
1010 1020 1030 1040 1050
GKFRLRGLLP GCVYHVQLKA EGNDHIERAL PHHRVIEVGN NDIDDVNIIV
1060 1070 1080 1090 1100
FRQINQFDLS GNVITSSEYL PTLWVKLYKS ENLDNPIQTV SLGQSLFFHF
1110 1120 1130 1140 1150
PPLLRDGENY VVLLDSTLPR SQYDYILPQV SFTAVGYHKH ITLIFNPTRK
1160 1170 1180 1190 1200
LPEQDIAQGS YIALPLTLLV LLAGYNHDKL IPLLLQLTSR LQGVRALGQA
1210 1220
ASDNSGPEDA KRQAKKQKTR RT
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