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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q4ACU6[1166 - 1166]
Description
Feature keyModified residue
Feature identifier
        10         20         30         40         50
MDGPGASAVV VRVGIPDLQQ TKCLRLDPTA PVWAAKQRVL CALNHSLQDA
60 70 80 90 100
LNYGLFQPPS RGRAGKFLDE ERLLQDYPPN LDTPLPYLEF RYKRRVYAQN
110 120 130 140 150
LIDDKQFAKL HTKANLKKFM DYVQLHSTDK VARLLDKGLD PNFHDPDSGE
160 170 180 190 200
CPLSLAAQLD NATDLLKVLR NGGAHLDFRT RDGLTAVHCA TRQRNAGALT
210 220 230 240 250
TLLDLGASPD YKDSRGLTPL YHSALGGGDA LCCELLLHDH AQLGTTDENG
260 270 280 290 300
WQEIHQACRF GHVQHLEHLL FYGANMGAQN ASGNTALHIC ALYNQESCAR
310 320 330 340 350
VLLFRGANKD VRNYNSQTAF QVAIIAGNFE LAEVIKTHKD SDVVPFRETP
360 370 380 390 400
SYAKRRRLAG PSGLASPRPL QRSASDINLK GDQPAASPGP TLRSLPHQLL
410 420 430 440 450
LQRLQEEKDR DRDGELENDI SGPSAGRGGH NKISPSGPGG SGPAPGPGPA
460 470 480 490 500
SPAPPAPPPR GPKRKLYSAV PGRKFIAVKA HSPQGEGEIP LHRGEAVKVL
510 520 530 540 550
SIGEGGFWEG TVKGRTGWFP ADCVEEVQMR QYDTRHETRE DRTKRLFRHY
560 570 580 590 600
TVGSYDSLTS HSDYVIDDKV AILQKRDHEG FGFVLRGAKA ETPIEEFTPT
610 620 630 640 650
PAFPALQYLE SVDVEGVAWR AGLRTGDFLI EVNGVNVVKV GHKQVVGLIR
660 670 680 690 700
QGGNRLVMKV VSVTRKPEED GARRRAPPPP KRAPSTTLTL RSKSMTAELE
710 720 730 740 750
ELASIRRRKG EKLDEILAVA AEPTLRPDIA DADSRAATVK QRPTSRRITP
760 770 780 790 800
AEISSLFERQ GLPGPEKLPG SLRKGIPRTK SVGEDEKLAS LLEGRFPRST
810 820 830 840 850
SMQDTVREGR GIPPPPQTAP PPPPAPYYFD SGPPPTFSPP PPPGRAYDTV
860 870 880 890 900
RSSFKPGLEA RLGAGAAGLY DPSTPLGPLP YPERQKRARS MIILQDSAPE
910 920 930 940 950
VGDVPRPAPA ATPPERPKRR PRPSGPDSPY ANLGAFSASL FAPSKPQRRK
960 970 980 990 1000
SPLVKQLQVE DAQERAALAV GSPGPVGGSF AREPSPTHRG PRPGSLDYSS
1010 1020 1030 1040 1050
GEGLGLTFGG PSPGPVKERR LEERRRSTVF LSVGAIEGSP PSADLPSLQP
1060 1070 1080 1090 1100
SRSIDERLLG TGATTGRDLL LPSPVSALKP LVGGPSLGPS GSTFIHPLTG
1110 1120 1130 1140 1150
KPLDPSSPLA LALAARERAL ASQTPSRSPT PVHSPDADRP GPLFVDVQTR
1160 1170 1180 1190 1200
DSERGPLASP AFSPRSPAWI PVPARREAEK PPREERKSPE DKKSMILSVL
1210 1220 1230 1240 1250
DTSLQRPAGL IVVHATSNGQ EPSRLGAEEE RPGTPELAPA PMQAAAVAEP
1260 1270 1280 1290 1300
MPSPRAQPPG SIPADPGPGQ GSSEEEPELV FAVNLPPAQL SSSDEETREE
1310 1320 1330 1340 1350
LARIGLVPPP EEFANGILLT TPPPGPGPLP TTVPSPASGK PSSELPPAPE
1360 1370 1380 1390 1400
SAADSGVEEA DTRSSSDPHL ETTSTISTVS SMSTLSSESG ELTDTHTSFA
1410 1420 1430 1440 1450
DGHTFLLEKP PVPPKPKLKS PLGKGPVTFR DPLLKQSSDS ELMAQQHHAA
1460 1470 1480 1490 1500
STGLASAAGP ARPRYLFQRR SKLWGDPVES RGLPGPEDDK PTVISELSSR
1510 1520 1530 1540 1550
LQQLNKDTRS LGEEPVGGLG SLLDPAKKSP IAAARLFSSL GELSTISAQR
1560 1570 1580 1590 1600
SPGGPGGGAS YSVRPSGRYP VARRAPSPVK PASLERVEGL GAGVGGAGRP
1610 1620 1630 1640 1650
FGLTPPTILK SSSLSIPHEP KEVRFVVRSV SARSRSPSPS PLPSPSPGSG
1660 1670 1680 1690 1700
PSAGPRRPFQ QKPLQLWSKF DVGDWLESIH LGEHRDRFED HEIEGAHLPA
1710 1720 1730
LTKEDFVELG VTRVGHRMNI ERALRQLDGS
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Main funding by: National Institutes of Health

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