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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q4ACU6-3
Description
Feature key
Feature identifier
        10         20         30         40         50
MEAPGAGFAC PLPPGIASVT YVFVYSPSGP GGSGPAPGPG PASPAPPAPP
60 70 80 90 100
PRGPKRKLYS AVPGRKFIAV KAHSPQGEGE IPLHRGEAVK VLSIGEGGFW
110 120 130 140 150
EGTVKGRTGW FPADCVEEVQ MRQYDTRHET REDRTKRLFR HYTVGSYDSL
160 170 180 190 200
TSHSDYVIDD KVAILQKRDH EGFGFVLRGA KAETPIEEFT PTPAFPALQY
210 220 230 240 250
LESVDVEGVA WRAGLRTGDF LIEVNGVNVV KVGHKQVVGL IRQGGNRLVM
260 270 280 290 300
KVVSVTRKPE EDGARRRAPP PPKRAPSTTL TLRSKSMTAE LEELASIRRR
310 320 330 340 350
KGEKLDEILA VAAEPTLRPD IADADSRAAT VKQRPTSRRI TPAEISSLFE
360 370 380 390 400
RQGLPGPEKL PGSLRKGIPR TKSVGEDEKL ASLLEGRFPR STSMQDTVRE
410 420 430 440 450
GRGIPPPPQT APPPPPAPYY FDSGPPPTFS PPPPPGRAYD TVRSSFKPGL
460 470 480 490 500
EARLGAGAAG LYDPSTPLGP LPYPERQKRA RSMIILQDSA PEVGDVPRPA
510 520 530 540 550
PAATPPERPK RRPRPSGPDS PYANLGAFSA SLFAPSKPQR RKSPLVKQLQ
560 570 580 590 600
VEDAQERAAL AVGSPGPVGG SFAREPSPTH RGPRPGSLDY SSGEGLGLTF
610 620 630 640 650
GGPSPGPVKE RRLEERRRST VFLSVGAIEG SPPSADLPSL QPSRSIDERL
660 670 680 690 700
LGTGATTGRD LLLPSPVSAL KPLVGGPSLG PSGSTFIHPL TGKPLDPSSP
710 720 730 740 750
LALALAARER ALASQTPSRS PTPVHSPDAD RPGPLFVDVQ TRDSERGPLA
760 770 780 790 800
SPAFSPRSPA WIPVPARREA EKPPREERKS PEDKKSMILS VLDTSLQRPA
810 820 830 840 850
GLIVVHATSN GQEPSRLGAE EERPGTPELA PAPMQAAAVA EPMPSPRAQP
860 870 880 890 900
PGSIPADPGP GQGSSEEEPE LVFAVNLPPA QLSSSDEETR EELARIGLVP
910 920 930 940 950
PPEEFANGIL LTTPPPGPGP LPTTVPSPAS GKPSSELPPA PESAADSGVE
960 970 980 990 1000
EADTRSSSDP HLETTSTIST VSSMSTLSSE SGELTDTHTS FADGHTFLLE
1010 1020 1030 1040 1050
KPPVPPKPKL KSPLGKGPVT FRDPLLKQSS DSELMAQQHH AASTGLASAA
1060 1070 1080 1090 1100
GPARPRYLFQ RRSKLWGDPV ESRGLPGPED DKPTVISELS SRLQQLNKDT
1110 1120 1130 1140 1150
RSLGEEPVGG LGSLLDPAKK SPIAAARLFS SLGELSTISA QRSPGGPGGG
1160 1170 1180 1190 1200
ASYSVRPSGR YPVARRAPSP VKPASLERVE GLGAGVGGAG RPFGLTPPTI
1210 1220 1230 1240 1250
LKSSSLSIPH EPKEVRFVVR SVSARSRSPS PSPLPSPSPG SGPSAGPRRP
1260 1270 1280 1290 1300
FQQKPLQLWS KFDVGDWLES IHLGEHRDRF EDHEIEGAHL PALTKEDFVE
1310 1320
LGVTRVGHRM NIERALRQLD GS
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Main funding by: National Institutes of Health

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