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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q54PU3[81 - 297]
Description
Feature keyCompositional bias
Feature identifier
        10         20         30         40         50
MKSDEEKSKE LKDSSKEILN GEKEQKMEVD EPKKDATASD STTADLENKK
60 70 80 90 100
ESSGSDDTAT TDVKKDDSKQ ENQTNDNNTS VNGKDEKEKT DEKGESKEEK
110 120 130 140 150
KETEKKVEEK KDETNVEEKP KQQIEEKVEE KKDEKKEQDK KEKEDEVMKD
160 170 180 190 200
QQDKDKEVEK EKEVEKEKEK EKEKEKEKEK EKEKEKEKEK EKEKEKEKEV
210 220 230 240 250
EKEIETKQVE KEIGEVEKET KKVENETKEV EKEKEKEMDK EKEKEEKSEE
260 270 280 290 300
KPQEKPQDKP QEKPEEKLEE KDNKNFENVD KEKKQEENVK NINGKEELSK
310 320 330 340 350
ESSPPIIDSS DNNNNNEKEN KNVDSNNSSN NNNNNNNNNN NNNNNNNNNN
360 370 380 390 400
NNGNDNDKNK KLENIINDDK EEMVIDESVS TKTDTNNNNN NNNNNNNTNG
410 420 430 440 450
TQEKGRDSPI NIESNSNIDL KNKEKTAPSP STPPNNSNTT TNNNNNNNNN
460 470 480 490 500
NNKEKQNGGE SYEEVDKSKI EEELEKKRQT MSDNIVVTSP KNVGSNGVTN
510 520 530 540 550
SVTDDDDDME EEYESVFAKD RNQEVSIAKS SYSANKEADL ETKKRLECLG
560 570 580 590 600
NMEKLEKEFT DLKEKFFNDK LTQLKKEIDE IKLGEHISFQ ERIRELENKK
610 620 630 640 650
DKKLMIAENW KNYQIQSIMN MYEAERQQYE DEHQEEATHL REKMVTTLSE
660 670 680 690 700
RQKRISEEKE SMRMVDNESR ITTRTRRRTQ QPNTQQQPVS VKKKQNPPSI
710 720
VYSLKENEIM EDYNLIFKTR R
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