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BLAST

About BLAST

<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q700S9[399 - 401]
Description
Feature keyBeta strand
Feature identifier
        10         20         30         40         50
MKLLSSWVVA ALAAQAAGAA ISHKLDGFTI REHADPAKRA LLQKYVTWDE 
        60         70         80         90        100
HSIFVNGERL MIFSGEVHPY RLPVASLYID IFEKVKALGF NCVSFYVDWA 
       110        120        130        140        150
LLEGNPGHYS AEGIFDLQPF FDAAKEAGIY LLARPGPYIN AEVSGGGFPG 
       160        170        180        190        200
WLQRVDGILR TSDEAYLKAT DNYASNIAAT IAKAQITNGG PIILYQPENE 
       210        220        230        240        250
YSGACCGYNG FPDGSYMQYI EDHARDAGIV VPFISNDAWA AGHNAPGTGA 
       260        270        280        290        300
GAVDIYGHDS YPLGFDCANP STWPSGNLPT YFHTSHEQQS PSTPYSLVEF 
       310        320        330        340        350
QGGAFDPWGG VGFAKCAALL NHEFERVFYK NDFSFGVAFL NLYMIFGGTN 
       360        370        380        390        400
WGNLGHPGGY TSYDYGSAIS ESRNITREKY SELKLLGNFA KVSPGYLVAN 
       410        420        430        440        450
PGDLSTSTYT NTADLTVTPL LGSNSSASSF FVIRHSDYSS QASVEYKLTV 
       460        470        480        490        500
PTSAGNLTIP QLGGSLTLSG RDSKIHVTDY DVAGTNILYS TAEVFTWKKF 
       510        520        530        540        550
NNEKVLVLYG GPGEHHEFAV SGASSSSVVE GSSSGISSKK VGKALVVAWD 
       560        570        580        590        600
VSTARRIVQV GSLKVFLLDR NSAYNYWVPQ VPTKGTAPGY SNQETTASSI 
       610        620        630        640        650
IVKAGYLVRS AYLDGNDLHI QADFNATTPI EVVGAPSGAK NLVINGKKTQ 
       660        670        680        690        700
TKVDKNGIWS ASVAYTAPKV QLPSLKSLKW KSVDTLPEAK NTYDDSAWTS 
       710        720        730        740        750
ADHAYTNNSA HSLQTPTSLF ASDYGYHTGA LLFRGHFTAN GKEKTFFVQT 
       760        770        780        790        800
KGGTAYGHSI WINETYVGSW AGTSINDNNN ATYTLPTLQS GKNYVITVVI 
       810        820        830        840        850
DNMGLDEDWT IGSEDMKNPR GIIQYSLSGQ EASAISWKLT GNLGGENYRD 
       860        870        880        890        900
TVRGPLNEGG LYAERQGFHQ PQPPTQKWDS SSPFTGLTKP GIRFYSTSFD 
       910        920        930        940        950
LDLPSGYDIP LYFNFGNSTS TPAAYRVQLY VNGYQYGKYV NNIGPQTSFP 
       960        970        980        990       1000
VPEGILNYHG TNWLALSLWA QEDNGAKLDS FELINTTPVL TSLGEVKSVN 
      1010 
QPKYQARKGA Y
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