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BLAST

About BLAST

<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q8AYS8-1
Description
Feature key
Feature identifier
        10         20         30         40         50
MSNNINANNL NTDSSSSPVN VPKMDALIIP VTMEVPCDSR GQRMWWAFLA 
        60         70         80         90        100
SSMVTFFGGL FIILLWRTLK YLWTVCCHCG VKNKEAQKIN GGGDTQADGA 
       110        120        130        140        150
CKPTDEKEEN VAAEVGWMTS VKDWAGVMIS AQTLTGRVLV VLVFALSIGA 
       160        170        180        190        200
LVIYFIDSSN PIESCQNFYK DFTLQIDMAF NVFFLLYFGL RFIAANDKLW 
       210        220        230        240        250
FWLEVNSVVD FFTVPPVFVS VYLNRSWLGL RFLRALRLIQ FSEILQFLNI 
       260        270        280        290        300
LKTSNSIKLV NLCSIFISTW LTAAGFIHLV ENSGDPWENF QNNQQLTYWE 
       310        320        330        340        350
CVYLLMVTMS TVGYGDVYAK TTLGRLFMVF FILGGLAMFA SYVPEIIELI 
       360        370        380        390        400
GNRKKYGGSY SAVSGRKHIV VCGHITLESV SNFLKDFLHK DRDDVNVEIV 
       410        420        430        440        450
FLHNISPNLE LEALFKRHFT QVEFYQGSVL NPHDLARVKI ESADACLILA 
       460        470        480        490        500
NKYCADPDAE DASNIMRVIS IKNYHPKIRI ITQMLQYHNK AHLLNIPSWN 
       510        520        530        540        550
WKEGDDAICL AELKLGFIAQ SCLAPGLSTM LANLFSMRSF IKIEEDTWQK 
       560        570        580        590        600
YYLEGVANEM YTEYLSSAFV GLSFPAVCEL VFAKLKLLMI AIEYKSEKRE 
       610        620        630        640        650
SSILINPGNH VKIQEGTLGF FIASDAKEVK RAFFYCKACH DDITDPKRIK 
       660        670        680        690        700
KCGCKRLEDE QPSTLSPKKK QRNGGMRNSP NSSPKLMRHD PLLIPGNEQI 
       710        720        730        740        750
DNMDANVKKY DSTGMFHWCP AKDIEKVILT RSEAAMTVLS GHVVVCIFGD 
       760        770        780        790        800
VKSALIGLRN LVMPLRASNF HYHELKHIVF VGSLEYLRRE WETLHNFPKV 
       810        820        830        840        850
SILPGTPLSR ADLRAVNINL CDMCVILSAN QNNIDDASLQ DKECILASLN 
       860        870        880        890        900
IKSMQFDDSI GVLQANSQGF TPPGMDRSSP DNSPVHGLLR QPSITTGANI 
       910        920        930        940        950
PIITELVNDS NVQFLDQDDD DDPDTELYLT QPFACGTAFA VSVLDSLMSA 
       960        970        980        990       1000
TYFNDNILTL IRTLVTGGAT PELEALIAEE NALRGGYSTP QTLANRDRCR 
      1010       1020       1030       1040       1050
VAQLALYDGP FADLGDGGCY GDLFCKALKT YNMLCFGIYR LRDAHLSTPS 
      1060       1070       1080       1090       1100
QCTKRYVITN PPYEFELVPT DLIFCLMQFD HNAGQSRASL SHSSHSSYSS 
      1110       1120       1130 
SKKSSSVHSI PSTANRPNRT KTRDSREKQK YVQEDRL
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