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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q9BZJ0-2
Description
Feature key
Feature identifier
        10         20         30         40         50
MAASTAAGKQ RIPKVAKVKN KAPAEVQITA EQLLREAKER ELELLPPPPQ
60 70 80 90 100
QKITDEEELN DYKLRKRKTF EDNIRKNRTV ISNWIKYAQW EESLKEIQRA
110 120 130 140 150
RSIYERALDV DYRNITLWLK YAEMEMKNRQ VNHARNIWDR AITTLPRVNQ
160 170 180 190 200
FWYKYTYMEE MLGNVAGARQ VFERWMEWQP EEQAWHSYIN FELRYKEVDR
210 220 230 240 250
ARTIYERFVL VHPDVKNWIK YARFEEKHAY FAHARKVYER AVEFFGDEHM
260 270 280 290 300
DEHLYVAFAK FEENQKEFER VRVIYKYALD RISKQDAQEL FKNYTIFEKK
310 320 330 340 350
FGDRRGIEDI IVSKRRFQYE EEVKANPHNY DAWFDYLRLV ESDAEAEAVR
360 370 380 390 400
EVYERAIANV PPIQEKRHWK RYIYLWINYA LYEELEAKDP ERTRQVYQAS
410 420 430 440 450
LELIPHKKFT FAKMWILYAQ FEIRQKNLSL ARRALGTSIG KCPKNKLFKV
460 470 480 490 500
YIELELQLRE FDRCRKLYEK FLEFGPENCT SWIKFAELET ILGDIDRARA
510 520 530 540 550
IYELAISQPR LDMPEVLWKS YIDFEIEQEE TERTRNLYRR LLQRTQHVKV
560 570 580 590 600
WISFAQFELS SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF
610 620 630 640 650
EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEEY FDYIFPEDAA
660 670 680
NQPNLKLLAM AKLWKKQQQE KEDAEHHPDE DVDESES
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