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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q9BZJ0-3
Description
Feature key
Feature identifier
        10         20         30         40         50
MTATVENLTF QKDTLGNAVD KNGRHGSTEP LVLAWSSQFR RLTWGCALDA
60 70 80 90 100
LHRSPCVAAS QHGVTHLIRS SRTPHSTRCR KEDAQPGHHG NGAASVTAQA
110 120 130 140 150
RGQRSVLQVP LPVPRSCLFS ESFVVSVSSQ SRFLASVPGT GVQRSTAADM
160 170 180 190 200
AASTAAGKQR IPKVAKVKNK APAEVQITAE QLLREAKERE LELLPPPPQQ
210 220 230 240 250
KITDEEELND YKLRKRKTFE DNIRKNRTVI SNWIKYAQWE ESLKEIQRAR
260 270 280 290 300
SIYERALDVD YRNITLWLKY AEMEMKNRQV NHARNIWDRA ITTLPRVNQF
310 320 330 340 350
WYKYTYMEEM LGNVAGARQV FERWMEWQPE EQAWHSYINF ELRYKEVDRA
360 370 380 390 400
RTIYERFVLV HPDVKNWIKY ARFEEKHAYF AHARKVYERA VEFFGDEHMD
410 420 430 440 450
EHLYVAFAKF EENQKEFERV RVIYKYALDR ISKQDAQELF KNYTIFEKKF
460 470 480 490 500
GDRRGIEDII VSKRRFQYEE EVKANPHNYD AWFDYLRLVE SDAEAEAVRE
510 520 530 540 550
VYERAIANVP PIQEKRHWKR YIYLWINYAL YEELEAKDPE RTRQVYQASL
560 570 580 590 600
ELIPHKKFTF AKMWILYAQF EIRQKNLSLA RRALGTSIGK CPKNKLFKVY
610 620 630 640 650
IELELQLREF DRCRKLYEKF LEFGPENCTS WIKFAELETI LGDIDRARAI
660 670 680 690 700
YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW
710 720 730 740 750
ISFAQFELSS GKEGSLTKCR QIYEEANKTM RNCEEKEERL MLLESWRSFE
760 770 780 790 800
EEFGTASDKE RVDKLMPEKV KKRRKVQTDD GSDAGWEEYF DYIFPEDAAN
810 820 830
QPNLKLLAMA KLWKKQQQEK EDAEHHPDED VDESES
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