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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

<td id="blast-numal">Limits the number of returned alignments.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Hitsi

Sequence feature

Entry & position(s)Q9Y5R2[156 - 645]
Description
Feature keyChain
Feature identifierPRO_0000028847
        10         20         30         40         50
MPRSRGGRAA PGPPPPPPPP GQAPRWSRWR VPGRLLLLLL PALCCLPGAA
60 70 80 90 100
RAAAAAAGAG NRAAVAVAVA RADEAEAPFA GQNWLKSYGY LLPYDSRASA
110 120 130 140 150
LHSAKALQSA VSTMQQFYGI PVTGVLDQTT IEWMKKPRCG VPDHPHLSRR
160 170 180 190 200
RRNKRYALTG QKWRQKHITY SIHNYTPKVG ELDTRKAIRQ AFDVWQKVTP
210 220 230 240 250
LTFEEVPYHE IKSDRKEADI MIFFASGFHG DSSPFDGEGG FLAHAYFPGP
260 270 280 290 300
GIGGDTHFDS DEPWTLGNAN HDGNDLFLVA VHELGHALGL EHSSDPSAIM
310 320 330 340 350
APFYQYMETH NFKLPQDDLQ GIQKIYGPPA EPLEPTRPLP TLPVRRIHSP
360 370 380 390 400
SERKHERQPR PPRPPLGDRP STPGTKPNIC DGNFNTVALF RGEMFVFKDR
410 420 430 440 450
WFWRLRNNRV QEGYPMQIEQ FWKGLPARID AAYERADGRF VFFKGDKYWV
460 470 480 490 500
FKEVTVEPGY PHSLGELGSC LPREGIDTAL RWEPVGKTYF FKGERYWRYS
510 520 530 540 550
EERRATDPGY PKPITVWKGI PQAPQGAFIS KEGYYTYFYK GRDYWKFDNQ
560 570 580 590 600
KLSVEPGYPR NILRDWMGCN QKEVERRKER RLPQDDVDIM VTINDVPGSV
610 620 630 640
NAVAVVIPCI LSLCILVLVY TIFQFKNKTG PQPVTYYKRP VQEWV
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