| http://purl.uniprot.org/citations/10024518 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10024518 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10024518 | http://www.w3.org/2000/01/rdf-schema#comment | "Complement receptor 2 (CR2) is important in the regulation of the B lymphocyte response; the regulation of its expression is therefore of central importance. We recently reported that a 42 kDa heterogeneous nuclear ribonucleoprotein (hnRNP) is involved in the transcriptional regulation of the human CR2 gene [Tolnay, Lambris and Tsokos (1997) J. Immunol. 159, 5492-5501]. We cloned the cDNA encoding this protein and found it to be identical with hnRNP D0B, a sequence-specific RNA-binding protein. By using a set of mutated oligonucleotides, we demonstrated that the recombinant hnRNP D0B displays sequence specificity for double-stranded oligonucleotide defined by the CR2 promoter. We conducted electrophoretic mobility-shift assays to estimate the apparent Kd of hnRNP D0B for the double-stranded DNA motif and found it to be 59 nM. Interestingly, hnRNP D0B displayed affinities of 28 and 18 nM for the sense and anti-sense strands of the CR2 promoter-defined oligonucleotide respectively. The significantly greater binding affinity of hnRNP D0B for single-stranded DNA than for double-stranded DNA suggests that the protein might melt the double helix. The intranuclear concentration of sequence-specific protein was estimated to be 250-400 nM, indicating that the protein binds to the CR2 promoter in vivo. Co-precipitation of a complex formed in vivo between hnRNP D0B and the TATA-binding protein demonstrates that hnRNP D0B interacts with the basal transcription apparatus. Our results suggest a new physiological role for hnRNP D0B that involves binding to double- and single-stranded DNA sequences in a specific manner and functioning as a transcription factor."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3380417"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3380417"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Tsokos G.C."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Tsokos G.C."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Tolnay M."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Tolnay M."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Vereshchagina L.A."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/author | "Vereshchagina L.A."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/pages | "417-425"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/pages | "417-425"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/title | "Heterogeneous nuclear ribonucleoprotein D0B is a sequence-specific DNA-binding protein."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/title | "Heterogeneous nuclear ribonucleoprotein D0B is a sequence-specific DNA-binding protein."xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/volume | "338"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://purl.uniprot.org/core/volume | "338"xsd:string |
| http://purl.uniprot.org/citations/10024518 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10024518 |
| http://purl.uniprot.org/citations/10024518 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10024518 |
| http://purl.uniprot.org/citations/10024518 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10024518 |
| http://purl.uniprot.org/citations/10024518 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10024518 |