| http://purl.uniprot.org/citations/10089347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10089347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10089347 | http://www.w3.org/2000/01/rdf-schema#comment | "Human lactoferrin (hLf) has considerable potential as a therapeutic agent. Overexpression of hLf in the fungus Aspergillus awamori has resulted in the availability of very large quantities of this protein. Here, the three-dimensional structure of the recombinant hLf has been determined by X-ray crystallography at a resolution of 2.2 A. The final model, comprising 5339 protein atoms (residues 1-691, 294 solvent molecules, two Fe3+and two CO32-ions), gives an R factor of 0.181 (free R = 0.274) after refinement against 32231 reflections in the resolution range 10-2.2 A. Superposition of the recombinant hLf structure onto the native milk hLf structure shows a very high level of correspondence; the main-chain atoms for the entire polypeptide can be superimposed with an r.m.s. deviation of only 0.3 A and there are no significant differences in side-chain conformations or in the iron-binding sites. Dynamic properties, as measured by B-value distributions or iron-release kinetics, also agree closely. This shows that the structure of the protein is not affected by the mode of expression, the use of strain-improvement procedures or the changes in glycosylation due to the fungal system."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444998011226"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444998011226"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Jameson G.B."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Jameson G.B."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Baker E.N."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Baker E.N."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Baker H.M."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Baker H.M."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Sun X.L."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Sun X.L."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Shewry S.C."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/author | "Shewry S.C."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/pages | "403-407"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/pages | "403-407"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/title | "Structure of recombinant human lactoferrin expressed in Aspergillus awamori."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/title | "Structure of recombinant human lactoferrin expressed in Aspergillus awamori."xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/10089347 | http://purl.uniprot.org/core/volume | "55"xsd:string |