| http://purl.uniprot.org/citations/10347185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10347185 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10347185 | http://www.w3.org/2000/01/rdf-schema#comment | "The functional role of neutrophils during acute inflammatory responses is regulated by two high affinity interleukin-8 receptors (CXCR1 and CXCR2) that are rapidly desensitized and internalized upon binding their cognate chemokine ligands. The efficient re-expression of CXCR1 on the surface of neutrophils following agonist-induced internalization suggests that CXCR1 surface receptor turnover may involve regulatory pathways and intracellular factors similar to those regulating beta2-adrenergic receptor internalization and re-expression. To examine the internalization pathway utilized by ligand-activated CXCR1, a CXCR1-GFP construct was transiently expressed in two different cell lines, HEK 293 and RBL-2H3 cells. While interleukin-8 stimulation promoted CXCR1 sequestration in RBL-2H3 cells, receptor internalization in HEK 293 cells required co-expression of G protein-coupled receptor kinase 2 and beta-arrestin proteins. The importance of beta-arrestins in CXCR1 internalization was confirmed by the ability of a dominant negative beta-arrestin 1-V53D mutant to block internalization of CXCR1 in RBL-2H3 cells. A role for dynamin was also demonstrated by the lack of CXCR1 internalization in dynamin I-K44A dominant negative mutant-transfected RBL-2H3 cells. Agonist-promoted co-localization of transferrin and CXCR1-GFP in endosomes of RBL-2H3 cells confirmed that receptor internalization occurs via clathrin-coated vesicles. Our data provides a direct link between agonist-induced internalization of CXCR1 and a requirement for G protein-coupled receptor kinase 2, beta-arrestins, and dynamin during this process."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.23.16287"xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.23.16287"xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Andrews J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Andrews J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Ferguson S.S.G."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Ferguson S.S.G."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Barlic J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Barlic J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "DeVries M.E."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "DeVries M.E."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Kelvin D.J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Kelvin D.J."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Khandaker M.H."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Khandaker M.H."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Mahon E."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Mahon E."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Mitchell G.B."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Mitchell G.B."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Rahimpour R."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Rahimpour R."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Tan C.M."xsd:string |
| http://purl.uniprot.org/citations/10347185 | http://purl.uniprot.org/core/author | "Tan C.M."xsd:string |