| http://purl.uniprot.org/citations/10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10375532 | http://www.w3.org/2000/01/rdf-schema#comment | "Many tumorigenic processes affect cell-cycle progression by their effects on the levels of the cyclin-dependent kinase inhibitor p27(Kip1) [1,2]. The phosphorylation- and ubiquitination-dependent proteolysis of p27 is implicated in control of the G1-S transition in the cell cycle [3-6]. To determine the factors that control p27 stability, we established a cell-free extract assay that recapitulates the degradation of p27. Phosphorylation of p27 at Thr187 was essential for its degradation. Degradation was also dependent on SCF(Skp2), a protein complex implicated in targeting phosphorylated proteins for ubiquitination [7-10]. Immunodepletion of components of the complex - Cul-1, Skp1, or Skp2 - from the extract abolished p27 degradation, while addition of purified SCF(Skp2) to Skp2-depleted extract restored the capacity to degrade p27. A specific association was observed between Skp2 and a p27 carboxy-terminal peptide containing phosphorylated Thr187, but not between Skp2 and the non-phosphorylated peptide. Skp2-dependent associations between Skp1 or Cul-1 and the p27 phosphopeptide were also detected. Isolated SCF(Skp2) contained an E3 ubiquitin ligase activity towards p27. Our data thus suggest that SCF(Skp2) specifically targets p27 for degradation during cell-cycle progression."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0960-9822(99)80290-5"xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/author | "Yeh K.H."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/author | "Tsvetkov L.M."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/name | "Curr Biol"xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/pages | "661-664"xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/title | "p27(Kip1) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27."xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/10375532 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10375532 |
| http://purl.uniprot.org/citations/10375532 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10375532 |
| http://purl.uniprot.org/uniprot/#_Q15008-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |
| http://purl.uniprot.org/uniprot/#_O43242-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |
| http://purl.uniprot.org/uniprot/#_P40306-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |
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| http://purl.uniprot.org/uniprot/#_Q13200-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |
| http://purl.uniprot.org/uniprot/#_O00231-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |
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| http://purl.uniprot.org/uniprot/#_O00233-mappedCitation-10375532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10375532 |