| http://purl.uniprot.org/citations/10449419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10449419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10449419 | http://www.w3.org/2000/01/rdf-schema#comment | "The 25S [U4/U6.U5] tri-snRNP (small nuclear ribonucleoprotein) is a central unit of the nuclear pre-mRNA splicing machinery. The U4, U5 and U6 snRNAs undergo numerous rearrangements in the spliceosome, and knowledge of all of the tri-snRNP proteins is crucial to the detailed investigation of the RNA dynamics during the spliceosomal cycle. Here we characterize by mass spectrometric methods the proteins of the purified [U4/U6.U5] tri-snRNP from the yeast Saccharomyces cerevisiae. In addition to the known tri-snRNP proteins (only one, Lsm3p, eluded detection), we identified eight previously uncharacterized proteins. These include four Sm-like proteins (Lsm2p, Lsm5p, Lsm6p and Lsm7p) and four specific proteins named Snu13p, Dib1p, Snu23p and Snu66p. Snu13p comprises a putative RNA-binding domain. Interestingly, the Schizosaccharomyces pombe orthologue of Dib1p, Dim1p, was previously assigned a role in cell cycle progression. The role of Snu23p, Snu66p and, additionally, Spp381p in pre-mRNA splicing was investigated in vitro and/or in vivo. Finally, we show that both tri-snRNPs and the U2 snRNP are co-precipitated with protein A-tagged versions of Snu23p, Snu66p and Spp381p from extracts fractionated by glycerol gradient centrifugation. This suggests that these proteins, at least in part, are also present in a [U2.U4/U6.U5] tetra-snRNP complex."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/18.16.4535"xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/18.16.4535"xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Mann M."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Mann M."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Neubauer G."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Neubauer G."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Fabrizio P."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Fabrizio P."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Gottschalk A."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Gottschalk A."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Banroques J."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/author | "Banroques J."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/pages | "4535-4548"xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/pages | "4535-4548"xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/title | "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."xsd:string |
| http://purl.uniprot.org/citations/10449419 | http://purl.uniprot.org/core/title | "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."xsd:string |