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http://purl.uniprot.org/citations/10469171http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10469171http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10469171http://www.w3.org/2000/01/rdf-schema#comment"

Background

Group 1 metabotropic glutamate receptors (mGluR1 and mGluR5) are coupled to inositol trisphosphate/Ca2+ signaling via G proteins and play an important role in excitatory synaptic transmission. To explore the regulation of group 1 mGluR function, we applied the yeast two-hybrid system using the intracellular carboxy-terminal domain of group 1 mGluRs (group 1 ct-mGluRs) and attempted to identify novel protein-protein interactions of group 1 mGluRs.

Results

The two-hybrid screening revealed a specific interaction between group 1 ct-mGluRs and Siah-1A, the mammalian homolog of Drosophila seven in absentia which is involved in photoreceptor cell differentiation via the ubiquitin/proteasome-dependent mechanism. This interaction occurs within a homologous 27-28 amino acid stretch within group 1 ct-mGluRs and requires the latter two-thirds of Siah-1A. Following coexpression in COS-7 cells, myc-tagged Siah-1A was coimmunoprecipitated with the flag-tagged ct-mGluR1 by anti-flag antibody. Furthermore, in vitro binding revealed that Siah-1A and Ca2+/calmodulin (CaM) binding sites overlap, such that Siah-1A binding is competitively inhibited by CaM in a Ca2+-dependent manner.

Conclusions

The results demonstrate a direct interaction between group 1 mGluRs and Siah-1A and suggest a novel modulatory mechanism mediated by a competitive interaction between Ca2+/CaM and Siah-1A."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1999.00269.x"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1999.00269.x"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Ishikawa K."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Ishikawa K."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nakanishi S."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nakanishi S."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Kaneko S."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Kaneko S."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Neki A."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Neki A."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nash S.R."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nash S.R."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nishimune A."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/author"Nishimune A."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/pages"381-390"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/pages"381-390"xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/title"Competitive interaction of seven in absentia homolog-1A and Ca2+/calmodulin with the cytoplasmic tail of group 1 metabotropic glutamate receptors."xsd:string
http://purl.uniprot.org/citations/10469171http://purl.uniprot.org/core/title"Competitive interaction of seven in absentia homolog-1A and Ca2+/calmodulin with the cytoplasmic tail of group 1 metabotropic glutamate receptors."xsd:string
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