| http://purl.uniprot.org/citations/10473609 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10473609 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10473609 | http://www.w3.org/2000/01/rdf-schema#comment | "SH2-B has been shown to be required for nerve growth factor (NGF)-mediated neuronal differentiation and survival, associate with NGF receptor TrkA, and be tyrosyl-phosphorylated in response to NGF. In this work, we examined whether NGF stimulates phosphorylation of SH2-B on serines/threonines. NGF promotes a dramatic upward shift in mobility of SH2-B, resulting in multiple forms that cannot be attributed to tyrosyl phosphorylation. Treatment of SH2-B with protein phosphatase 2A, a serine/threonine phosphatase, reduces the many forms to two. PD98059, a MEK inhibitor, dramatically inhibits NGF-promoted phosphorylation of SH2-B on serines/threonines, whereas depletion of 4beta-phorbol 12-myristate 13-acetate-sensitive protein kinase Cs does not. ERKs 1 and 2 phosphorylate SH2-Bbeta primarily on Ser-96 in vitro. However, NGF still stimulates serine/threonine phosphorylation of SH2-Bbeta(S96A). SH2-Bbeta(S96A), like wild-type SH2-Bbeta, enhances NGF-induced neurite outgrowth. In contrast, SH2-Bbeta(R555E) containing a defective SH2 domain blocks NGF-induced neurite outgrowth and displays greatly reduced phosphorylation on serines/threonines in response to NGF. SH2-Bbeta(R555E), like wild-type SH2-Bbeta, associates with the plasma membrane, suggesting that the dominant negative effect of SH2-Bbeta(R555E) cannot be explained by an abnormal subcellular distribution. In summary, NGF stimulates phosphorylation of SH2-B on serines/threonines by kinases downstream of MEK, which may be important for NGF-mediated neuronal differentiation and survival."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.37.26485"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.37.26485"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Rui L."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Rui L."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Carter-Su C."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Carter-Su C."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Herrington J."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/author | "Herrington J."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/pages | "26485-26492"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/pages | "26485-26492"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/title | "SH2-B, a membrane-associated adapter, is phosphorylated on multiple serines/threonines in response to nerve growth factor by kinases within the MEK/ERK cascade."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/title | "SH2-B, a membrane-associated adapter, is phosphorylated on multiple serines/threonines in response to nerve growth factor by kinases within the MEK/ERK cascade."xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10473609 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10473609 |
| http://purl.uniprot.org/citations/10473609 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10473609 |
| http://purl.uniprot.org/citations/10473609 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10473609 |
| http://purl.uniprot.org/citations/10473609 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10473609 |