| http://purl.uniprot.org/citations/10490027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10490027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10490027 | http://www.w3.org/2000/01/rdf-schema#comment | "Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal regulated kinases (ERK). This kinase cascade controls the proliferation and differentiation of different cell types. Here we describe a Raf-1-interacting protein, isolated using a yeast two-hybrid screen. This protein inhibits the phosphorylation and activation of MEK by Raf-1 and is designated RKIP (Raf kinase inhibitor protein). In vitro, RKIP binds to Raf-1, MEK and ERK, but not to Ras. RKIP co-immunoprecipitates with Raf-1 and MEK from cell lysates and colocalizes with Raf-1 when examined by confocal microscopy. RKIP is not a substrate for Raf-1 or MEK, but competitively disrupts the interaction between these kinases. RKIP overexpression interferes with the activation of MEK and ERK, induction of AP-1-dependent reporter genes and transformation elicited by an oncogenically activated Raf-1 kinase. Downregulation of endogenous RKIP by expression of antisense RNA or antibody microinjection induces the activation of MEK-, ERK- and AP-1-dependent transcription. RKIP represents a new class of protein-kinase-inhibitor protein that regulates the activity of the Raf/MEK/ERK module."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.org/dc/terms/identifier | "doi:10.1038/43686"xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.org/dc/terms/identifier | "doi:10.1038/43686"xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Kolch W."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Kolch W."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Mischak H."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Mischak H."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Rose D.W."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Rose D.W."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Katsanakis K.D."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Katsanakis K.D."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Yeung K."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Yeung K."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Sedivy J.M."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Sedivy J.M."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Kaiser C."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Kaiser C."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Fee F."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Fee F."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Janosch P."xsd:string |
| http://purl.uniprot.org/citations/10490027 | http://purl.uniprot.org/core/author | "Janosch P."xsd:string |