| http://purl.uniprot.org/citations/10614647 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10614647 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10614647 | http://www.w3.org/2000/01/rdf-schema#comment | "Recently, we cloned a messenger RNA (mRNA) predominantly expressed in chondrocytes from a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8, by differential display PCR and found that its gene, named hcs24, was identical with that of connective tissue growth factor (CTGF). Here we investigated CTGF/Hcs24 function in the chondrocytic cell line HCS-2/8 and rabbit growth cartilage (RGC) cells. HCS-2/8 cells transfected with recombinant adenoviruses that generate CTGF/Hcs24 sense RNA (mRNA) proliferated more rapidly than HCS-2/8 cells transfected with control adenoviruses. HCS-2/8 cells transfected with recombinant adenoviruses that generate CTGF/Hcs24 sense RNA expressed more mRNA of aggrecan and type X collagen than the control cells. To elucidate the direct action of CTGF/Hcs24 on the cells, we transfected HeLa cells with CTGF/Hcs24 expression vectors, obtained stable transfectants, and purified recombinant CTGF/Hcs24 protein from conditioned medium of the transfectants. The recombinant CTGF/Hcs24 effectively promoted the proliferation of HCS-2/8 cells and RGC cells in a dose-dependent manner and also dose dependently increased proteoglycan synthesis in these cells. In addition, these stimulatory effects of CTGF/Hcs24 were neutralized by the addition of anti-CTGF antibodies. Furthermore, the recombinant CTGF/Hcs24 effectively increased alkaline phosphatase activity in RGC cells in culture. Moreover, RT-PCR analysis revealed that the recombinant CTGF/Hcs24 stimulated gene expression of aggrecan and collagen types II and X in RGC cells in culture. These results indicate that CTGF/Hcs24 directly promotes the proliferation and differentiation of chondrocytes."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.141.1.7267"xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.141.1.7267"xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Kubo T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Kubo T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Nakanishi T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Nakanishi T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Nishida T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Nishida T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Takigawa M."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Takigawa M."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Tezuka K."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Tezuka K."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Shimo T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Shimo T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Tamatani T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/author | "Tamatani T."xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/10614647 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |