| http://purl.uniprot.org/citations/10681522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10681522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10681522 | http://www.w3.org/2000/01/rdf-schema#comment | "Tyrosine phosphorylation of membrane proteins plays a crucial role in cell signaling by recruiting Src homology 2 (SH2) domain-containing signaling molecules. Recently, we have isolated a transmembrane protein designated PZR that specifically binds tyrosine phosphatase SHP-2, which has two SH2 domains (Zhao, Z. J., and Zhao, R. (1998) J. Biol. Chem. 273, 29367-29372). PZR belongs to the immunoglobulin superfamily. Its intracellular segment contains four putative sites of tyrosine phosphorylation. By site-specific mutagenesis, we found that the tyrosine 241 and 263 embedded in the consensus immunoreceptor tyrosine-based inhibitory motifs VIYAQL and VVYADI, respectively, accounted for the entire tyrosine phosphorylation of PZR. The interaction between PZR and SHP-2 requires involvement of both tyrosyl residues of the former and both SH2 domains of the latter, since its was disrupted by mutating a single tyrosyl residue or an SH2 domain. Overexpression of catalytically inactive but not active forms of SHP-2 bearing intact SH2 domains in cells caused hyperphosphorylation of PZR. In vitro, tyrosine-phosphorylated PZR was efficiently dephosphorylated by the full-length form of SHP-2 but not by its SH2 domain-truncated form. Together, the data indicate that PZR serves not only as a specific anchor protein of SHP-2 on the plasma membrane but also as a physiological substrate of the enzyme. The coexisting binding and dephosphorylation of PZR by SHP-2 may function to terminate signal transduction initiated by PZR and SHP-2 and to set a threshold for the signal transduction to be initiated."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.8.5453"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.8.5453"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/author | "Zhao R."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/author | "Zhao R."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/author | "Zhao Z.J."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/author | "Zhao Z.J."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/pages | "5453-5459"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/pages | "5453-5459"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/title | "Dissecting the interaction of SHP-2 with PZR, an immunoglobulin family protein containing immunoreceptor tyrosine-based inhibitory motifs."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/title | "Dissecting the interaction of SHP-2 with PZR, an immunoglobulin family protein containing immunoreceptor tyrosine-based inhibitory motifs."xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10681522 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10681522 |
| http://purl.uniprot.org/citations/10681522 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10681522 |
| http://purl.uniprot.org/citations/10681522 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10681522 |
| http://purl.uniprot.org/citations/10681522 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10681522 |
| http://purl.uniprot.org/uniprot/O95297 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10681522 |
| http://purl.uniprot.org/uniprot/Q06124 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10681522 |