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http://purl.uniprot.org/citations/10722751http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10722751http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10722751http://www.w3.org/2000/01/rdf-schema#comment"Previous studies have provided detailed information on the formation of spherical high density lipoproteins (HDL) containing apolipoprotein (apo) A-I but no apoA-II (A-I HDL) by an lecithin:cholesterol acyltransferase (LCAT)-mediated process. In this study we have investigated the formation of spherical HDL containing both apoA-I and apoA-II (A-I/A-II HDL). Incubations were carried out containing discoidal A-I reconstituted HDL (rHDL), discoidal A-II rHDL, and low density lipoproteins in the absence or presence of LCAT. After the incubation, the rHDL were reisolated and subjected to immunoaffinity chromatography to determine whether A-I/A-II rHDL were formed. In the absence of LCAT, the majority of the rHDL remained as either A-I rHDL or A-II rHDL, with only a small amount of A-I/A-II rHDL present. By contrast, when LCAT was present, a substantial proportion of the reisolated rHDL were A-I/A-II rHDL. The identity of the particles was confirmed using apoA-I rocket electrophoresis. The formation of the A-I/A-II rHDL was influenced by the relative concentrations of the precursor discoidal A-I and A-II rHDL. The A-I/A-II rHDL included several populations of HDL-sized particles; the predominant population having a Stokes' diameter of 9.9 nm. The particles were spherical in shape and had an electrophoretic mobility slightly slower than that of the alpha-migrating HDL in human plasma. The apoA-I:apoA-II molar ratio of the A-I/A-II rHDL was 0.7:1. Their major lipid constituents were phospholipids, unesterified cholesterol, and cholesteryl esters. The results presented are consistent with LCAT promoting fusion of the A-I rHDL and A-II rHDL to form spherical A-I/A-II rHDL. We suggest that this process may be an important source of A-I/A-II HDL in human plasma."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.org/dc/terms/identifier"doi:10.1074/jbc.275.12.9019"xsd:string
http://purl.uniprot.org/citations/10722751http://purl.org/dc/terms/identifier"doi:10.1074/jbc.275.12.9019"xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Barter P.J."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Barter P.J."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Clay M.A."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Clay M.A."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Pyle D.H."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Pyle D.H."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Rye K.A."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/author"Rye K.A."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/pages"9019-9025"xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/pages"9019-9025"xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/title"Formation of spherical, reconstituted high density lipoproteins containing both apolipoproteins A-I and A-II is mediated by lecithin:cholesterol acyltransferase."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/title"Formation of spherical, reconstituted high density lipoproteins containing both apolipoproteins A-I and A-II is mediated by lecithin:cholesterol acyltransferase."xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/volume"275"xsd:string
http://purl.uniprot.org/citations/10722751http://purl.uniprot.org/core/volume"275"xsd:string
http://purl.uniprot.org/citations/10722751http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10722751
http://purl.uniprot.org/citations/10722751http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10722751
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