| http://purl.uniprot.org/citations/10769138 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10769138 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10769138 | http://www.w3.org/2000/01/rdf-schema#comment | "On the basis of (13)C and deuterium isotope effects, L-ribulose-5-phosphate 4-epimerase catalyzes the epimerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate by an aldol cleavage to the enediolate of dihydroxyacetone and glycolaldehyde phosphate, followed by rotation of the aldehyde group and condensation to the epimer at C-4. With the wild-type enzyme, (13)C isotope effects were 1.85% at C-3 and 1.5% at C-4 at pH 7, with the values increasing to 2.53 and 2.05% at pH 5.5, respectively. H97N and Y229F mutants at pH 7 gave values of 3.25 and 2.53% at C-3 and 2. 69 and 1.99% at C-4, respectively. Secondary deuterium isotope effects at C-3 were 2.5% at pH 7 and 3.1% at pH 5.5 with the wild-type enzyme, and 4.1% at pH 7 with H97N. At C-4, the corresponding values were 9.6, 14, and 19%. These data suggest that H97N shows no commitments, while the wild-type enzyme has an external commitment of approximately 1.4 at pH 7 and an internal commitment independent of pH of approximately 0.6. The Y229 mutant shows only the internal commitment of 0.6. The sequence of the epimerase is similar to those of L-fuculose-1-phosphate and L-rhamnulose-1-phosphate aldolases for residues in the active site of L-fuculose-1-phosphate aldolase, suggesting that Asp76, His95, His97, and His171 of the epimerase may be metal ion ligands, and Ser44, Gly45, Ser74, and Ser75 may form a phosphate binding pocket. The pH profile of V/K for L-ribulose 5-phosphate is bell-shaped with pK values of 5.94 and 8.24. The CD spectra of L-ribulose 5-phosphate and D-xylulose 5-phosphate differ sufficiently that the epimerization reaction can be followed at 300 nm."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi992894+"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi992894+"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Cleland W.W."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Cleland W.W."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Lee L.V."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Lee L.V."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Vu M.V."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/author | "Vu M.V."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/pages | "4808-4820"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/pages | "4808-4820"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/title | "13C and deuterium isotope effects suggest an aldol cleavage mechanism for L-ribulose-5-phosphate 4-epimerase."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/title | "13C and deuterium isotope effects suggest an aldol cleavage mechanism for L-ribulose-5-phosphate 4-epimerase."xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/10769138 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10769138 |
| http://purl.uniprot.org/citations/10769138 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10769138 |
| http://purl.uniprot.org/citations/10769138 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10769138 |
| http://purl.uniprot.org/citations/10769138 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10769138 |