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http://purl.uniprot.org/citations/10827258http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10827258http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10827258http://www.w3.org/2000/01/rdf-schema#comment"

Background

Antigens of the MNS blood group system are located on two sialoglycoproteins, GPA and GPB, encoded by GYPA and GYPB. The molecular backgrounds of the low-frequency antigens Ny(a) and Os(a) are not known.

Study design and methods

Immunoblotting and a monoclonal antibody-specific immobilization of erythrocyte antigens (MAIEA) assay were used to analyze Os(a). PCR-amplified products of the coding exons of GYPA were studied by single-strand conformation polymorphism analysis, and exon 3 was sequenced. Synthetic peptides were used in hemagglutination-inhibition tests.

Results

Sequencing of GYPA exon 3 of two unrelated Ny(a+) persons revealed heterozygosity for a T194A base change encoding an Asp27Glu substitution. Immunoblotting with anti-Os(a) and an MAIEA assay with MoAbs to GPA showed that Os(a) is on GPA. Sequencing exon 3 of an Os(a+) person from the only family with Os(a) revealed heterozygosity for a C273T base change encoding a Pro54Ser substitution. A synthetic peptide representing part of GPA with the Os(a) mutation (VRTVYPSEEETGE) completely inhibited anti-Os(a), whereas the control peptide (VRTVYPPEEETGE) did not inhibit anti-Os(a).

Conclusion

Ny(a) and Os(a) are low-frequency antigens of the MNS blood group system that represent Asp27Glu and Pro54Ser substitutions in GPA, respectively."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.org/dc/terms/identifier"doi:10.1046/j.1537-2995.2000.40050555.x"xsd:string
http://purl.uniprot.org/citations/10827258http://purl.org/dc/terms/identifier"doi:10.1046/j.1537-2995.2000.40050555.x"xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Okubo Y."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Okubo Y."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Bruce L.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Bruce L.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Tanner M.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Tanner M.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Mawby W.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Mawby W.J."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Daniels G.L."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Daniels G.L."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Green C.A."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Green C.A."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Petty A."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Petty A."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Kornstad L."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/author"Kornstad L."xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/name"Transfusion"xsd:string
http://purl.uniprot.org/citations/10827258http://purl.uniprot.org/core/name"Transfusion"xsd:string
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