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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/10860554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10860554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10860554 | http://www.w3.org/2000/01/rdf-schema#comment | "The human liver CYP4F2 gene (Accession No. AF221943) encodes a leukotriene B(4) omega-hydroxylase that metabolizes leukotriene B(4) (LTB(4)) to a less potent proinflammatory eicosanoid, 20-OH-LTB(4). We sequenced a 6.7-kb genomic fragment of the human CYP4F2 gene that has the first five exons and 500 bp of the 5'-flanking region. The major transcription start site was found to be 49 bp upstream of the 3' end of exon 1 and the ATG translation initiation codon was located in exon 2. Besides the TATA box at -39 bp and basal transcription factor binding sites, the promoter region and 412-bp intron 1 have several putative binding sites for nuclear factors that may mediate the inflammatory response and lipid homeostasis. We found two DR1 elements in the 5' promoter, a DR2 element in intron 1, and RXR/RAR binding sites in both intron 1 and the 5' promoter. DNase I footprinting revealed three protected sequences, with the region containing two CAATT boxes at -71 and -111 bp important in CYP4F2 gene expression. Luciferase reporter assays showed that the 500-bp upstream sequence has strong promoter activity. Transient transfection experiments identified two sites in the 5' promoter and intron 1 that cooperate in gene transcription while exon 1 and a GC-rich region flanking exon 1 inhibit transcription. trans-Retinoic acid and 9-cis-retinoic acid stimulate promoter activity 3- and 6-fold, respectively, while cotransfection with RXRalpha or RAR/RXRalpha further enhanced activity. Peroxisome proliferators inhibit CYP4F2 gene promoter activity and cotransfection with PPARalpha or PPARalpha/RXRalpha can slightly attenuate this inhibition. Both saturated fatty acids and 12-hydroxydodecanoic acid (12-OH-C(12)) can stimulate CYP4F2 gene promoter activity. Therefore, the CYP4F2 gene is repressed by peroxisomal proliferators and induced by retinoic acid, with RAR/RXRalpha mediating the induction while PPARalpha/RXR functions neither in the repression nor in the induction by peroxisomal proliferators or retinoic acid."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.org/dc/terms/identifier | "doi:10.1006/abbi.2000.1836"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.org/dc/terms/identifier | "doi:10.1006/abbi.2000.1836"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Hardwick J.P."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/author | "Hardwick J.P."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/pages | "364-376"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/pages | "364-376"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/title | "Promoter activity and regulation of the CYP4F2 leukotriene B4 omega hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/title | "Promoter activity and regulation of the CYP4F2 leukotriene B4 omega hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells."xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/volume | "378"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://purl.uniprot.org/core/volume | "378"xsd:string |
| http://purl.uniprot.org/citations/10860554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10860554 |
| http://purl.uniprot.org/citations/10860554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10860554 |
| http://purl.uniprot.org/citations/10860554 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10860554 |
| http://purl.uniprot.org/citations/10860554 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10860554 |