| http://purl.uniprot.org/citations/10861294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10861294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10861294 | http://www.w3.org/2000/01/rdf-schema#comment | "The human AF9 gene at 9p22 is one of the most common fusion partner genes with the MLL gene at 11q23, resulting in the t(9;11)(p22;q23). The MLL-AF9 fusion gene is associated with de novo acute myelo-genous leukemia (AML), rarely with acute lymphocytic leukemia (ALL) and with therapy related leukemia (t-AML). The AF9 gene is >100 kb and two patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Patient breakpoint locations were determined previously by RT-PCR and by genomic DNA cloning. In this study, we defined the exon-intron boundaries and identified several different structural elements in AF9 including a co-localizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. The location of the in vivo topo II cleavage site was confirmed in vitro using a topo II cleavage assay. In addition, two scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border both patient breakpoint regions: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. This study demonstrates that the patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. We describe a DNA breakage and repair model for non-homologous recombination between MLL and its partner genes, particularly AF9."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/9.11.1671"xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/9.11.1671"xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Roe B.A."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Roe B.A."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Rowley J.D."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Rowley J.D."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Strick R."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Strick R."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Strissel P.L."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Strissel P.L."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Tomek R.J."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Tomek R.J."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Zeleznik-Le N.J."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/author | "Zeleznik-Le N.J."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/pages | "1671-1679"xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/pages | "1671-1679"xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/title | "DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis."xsd:string |
| http://purl.uniprot.org/citations/10861294 | http://purl.uniprot.org/core/title | "DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis."xsd:string |