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http://purl.uniprot.org/citations/10873800http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10873800http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10873800http://www.w3.org/2000/01/rdf-schema#comment"

Background

Dictyostelium Akt/PKB is homologous to mammalian Akt/PKB and is required for cell polarity and proper chemotaxis during early development. The kinase activity of Akt/PKB kinase is activated in response to chemoattractants in neutrophils and in Dictyostelium by the chemoattractant cAMP functioning via a pathway involving a heterotrimeric G protein and PI3-kinase. Dictyostelium contains several kinases structurally related to Akt/PKB, one of which, PKBR-1, is investigated here for its role in cell polarity, movement and cellular morphogenesis during development.

Results

PKBR-1 has a kinase and a carboxy-terminal domain related to those of Akt/PKB, but no PH domain. Instead, it has an amino-terminal myristoylation site, which is required for its constitutive membrane localization. Like Akt/PKB, PKBR-1 is activated by cAMP through a G-protein-dependent pathway, but does not require PI3-kinase, probably because of the constitutive membrane localization of PKBR-1. This is supported by experiments demonstrating the requirement for membrane association for activation and in vivo function of PKBR-1. PKBR-1 protein is found in all cells throughout early development but is then restricted to the apical cells in developing aggregates, which are thought to control morphogenesis. PKBR-1 null cells arrest development at the mound stage and are defective in morphogenesis and multicellular development. These phenotypes are complemented by Akt/PKB, suggesting functional overlap between PKBR-1 and Akt/PKB. Akt/PKB PKBR-1 double knockout cells exhibit growth defects and show stronger chemotaxis and cell-polarity defects than Akt/PKB null cells.

Conclusions

Our results expand the previously known functions of Akt/PKB family members in cell movement and morphogenesis during Dictyostelium multicellular development. The results suggest that Akt/PKB and PKBR-1 have overlapping effectors and biological function: Akt/PKB functions predominantly during aggregation to control cell polarity and chemotaxis, whereas PKBR-1 is required for morphogenesis during multicellular development."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.org/dc/terms/identifier"doi:10.1016/s0960-9822(00)00536-4"xsd:string
http://purl.uniprot.org/citations/10873800http://purl.org/dc/terms/identifier"doi:10.1016/s0960-9822(00)00536-4"xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Firtel R.A."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Firtel R.A."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Ellsworth C."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Ellsworth C."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Meili R."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/author"Meili R."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/pages"708-717"xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/pages"708-717"xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/title"A novel Akt/PKB-related kinase is essential for morphogenesis in Dictyostelium."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/title"A novel Akt/PKB-related kinase is essential for morphogenesis in Dictyostelium."xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/10873800http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/10873800http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10873800
http://purl.uniprot.org/citations/10873800http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10873800
http://purl.uniprot.org/citations/10873800http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10873800
http://purl.uniprot.org/citations/10873800http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10873800
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