| http://purl.uniprot.org/citations/10880251 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10880251 | http://www.w3.org/2000/01/rdf-schema#comment | "The purpose of this study was to determine if interleukin 8 (IL-8) in complex with alpha2-macroglobulin (alpha-2-M) can be taken up by human alveolar macrophages. First, we demonstrated that human alveolar macrophages have receptors for alpha-2-M but not IL-8. The binding of(125)I-labeled alpha-2-M to the cells was specific and saturable, whereas(125)I-labeled recombinant human IL-8 (rhIL-8) did not bind to macrophages. However,(125)I-rhIL-8-alpha-2-M complexes bound to macrophages, and unlabeled alpha-2-M competed for the binding. We then cultured the cells in the presence of(125)I-rhIL-8-alpha-2-M complexes,(125)I-rhIL-8 alone or buffer for 24 h. Macrophages were lysed, and the released radioactivity measured. IL-8 concentrations in supernatants and cells were also measured using an IL-8 ELISA. When the macrophages were incubated with(125)I-rhIL-8-alpha-2-M complexes there was a significant amount of IL-8 associated with the cells. However, this was not the case when the cells were incubated with(125)I-rhIL-8 alone suggesting that only these complexes were taken-up by human alveolar macrophages. Furthermore, the clearance of complexes was specifically inhibited by a monoclonal antibody against the 515-kDa subunit of the alpha-2-M receptor (alpha-2-MR) but not by an isotopic mouse IgG1. The study shows an important clearance mechanism for IL-8 in the lung."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.org/dc/terms/identifier | "doi:10.1006/cyto.1999.0640"xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/author | "Alden S.M."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/author | "Carr F.K."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/author | "Kurdowska A."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/author | "Noble J.M."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/author | "Stevens M.D."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/name | "Cytokine"xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/pages | "1046-1053"xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/title | "Involvement of alpha-2-macroglobulin receptor in clearance of interleukin 8-alpha-2-macroglobulin complexes by human alveolar macrophages."xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/10880251 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10880251 |
| http://purl.uniprot.org/citations/10880251 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10880251 |
| http://purl.uniprot.org/uniprot/P01023#attribution-F785263467FFF2DCC59237AC9D9F54E6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10880251 |
| http://purl.uniprot.org/uniprot/Q07954#attribution-D94C1A3DEC7544653EB8F57CF9427FD4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10880251 |