| http://purl.uniprot.org/citations/10887191 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10887191 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10887191 | http://www.w3.org/2000/01/rdf-schema#comment | "The BRCA1 COOH terminus (BRCT) motif is present in many nuclear proteins that contribute to cell cycle regulation or DNA repair. Polymerase chain reaction-based screening with degenerate primers targeted to the BRCT motif resulted in the isolation of a human cDNA for a previously unidentified DNA polymerase (designated DNA polymerase beta2) that is closely related to DNA polymerase beta (Pol beta). The predicted Pol beta2 protein contains a BRCT motif in its NH(2)-terminal region; its COOH-terminal region exhibits 33% sequence identity to a corresponding region of human Pol beta. The Pol beta2 gene is expressed in a tissue-specific manner, with transcripts being most abundant in testis. A fusion construct comprising Pol beta2 and green fluorescent protein exhibited a predominantly nuclear localization in transfected HeLa cells. Recombinant human Pol beta2 from insect cells exhibited substantial DNA polymerase activity, but it did not possess terminal deoxyribonucleotidyl transferase activity. A truncated Pol beta2 mutant lacking the BRCT motif retained substantial DNA polymerase activity, whereas a mutant Pol beta2 with two alanine point mutations within the DNA polymerase active site did not. These results indicate that Pol beta2 is a Pol beta-related DNA polymerase with a BRCT motif that is dispensable for its polymerase activity."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m004263200"xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m004263200"xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Hirai M."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Hirai M."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Ikeda K."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Ikeda K."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nakanishi M."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nakanishi M."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Kitamura K."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Kitamura K."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nimura Y."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nimura Y."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Yasui A."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Yasui A."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Matsukage A."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Matsukage A."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nagasawa K.I."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/author | "Nagasawa K.I."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10887191 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |