| http://purl.uniprot.org/citations/10891506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10891506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10891506 | http://www.w3.org/2000/01/rdf-schema#comment | "Cse4p is a structural component of the core centromere of Saccharomyces cerevisiae and is a member of the conserved CENP-A family of specialized histone H3 variants. The histone H4 allele hhf1-20 confers defects in core centromere chromatin structure and mitotic chromosome transmission. We have proposed that Cse4p and histone H4 interact through their respective histone fold domains to assemble a nucleosome-like structure at centromeric DNA. To test this model, we targeted random mutations to the Cse4p histone fold domain and isolated three temperature-sensitive cse4 alleles in an unbiased genetic screen. Two of the cse4 alleles contain mutations at the Cse4p-H4 interface. One of these requires two widely separated mutations demonstrating long-range cooperative interactions in the structure. The third cse4 allele is mutated at its helix 2-helix 3 interface, a region required for homotypic H3 fold dimerization. Overexpression of wild-type Cse4p and histone H4 confer reciprocal allele-specific suppression of cse4 and hhf1 mutations, providing strong evidence for Cse4p-H4 protein interaction. Overexpression of histone H3 is dosage lethal in cse4 mutants, suggesting that histone H3 competes with Cse4p for histone H4 binding. However, the relative resistance of the Cse4p-H4 pathway to H3 interference argues that centromere chromatin assembly must be highly regulated."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.15.5700-5711.2000"xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.15.5700-5711.2000"xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Smith M.M."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Smith M.M."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Glowczewski L."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Glowczewski L."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Kalashnikova T."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Kalashnikova T."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Santisteban M.S."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/author | "Santisteban M.S."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/pages | "5700-5711"xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/pages | "5700-5711"xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/title | "Histone-histone interactions and centromere function."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/title | "Histone-histone interactions and centromere function."xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10891506 | http://purl.uniprot.org/core/volume | "20"xsd:string |