| http://purl.uniprot.org/citations/10912628 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10912628 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10912628 | http://www.w3.org/2000/01/rdf-schema#comment | "Glycophorin A (GPA), the major sialoglycoprotein of human erythrocyte membranes, was isolated separately from blood group A and B erythrocytes using phenol-water extraction. After purification, performed as gel filtration in the presence of SDS, two glycophorin samples GPA-A and GPA-B were run, in duplicate, in SDS-PAGE and electroblotted onto Immobilon P. After staining with 1) anti-glycophorin antibody and 2) with relevant anti-blood group (A or B) antibody it was shown that the band pattern of the samples in each duplicate was the same. GPA-A and GPA-B samples were also degraded using Carlson degradation (beta-elimination in mild alkaline/strong reducing conditions) and from reaction products the fractions of O-glycans and N-glycans were isolated; they were used in hemagglutination inhibition test. It was shown that both sugar fractions derived from GPA-A did inhibit agglutination of blood group A erythrocytes by anti-A antibody, whereas oligosaccharide fractions derived from GPA-B inhibited agglutination of blood group B erythrocytes by anti-B antibody. These results, obtained using immunochemical methods, confirm the presence of blood group A and B determinants in the carbohydrate moiety of human glycophorin, derived from the blood group A or B erythrocytes, respectively."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/author | "Krotkiewski H."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/author | "Krotkiewski H."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/author | "Podbielska M."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/author | "Podbielska M."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/name | "Arch. Immunol. Ther. Exp."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/name | "Arch. Immunol. Ther. Exp."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/pages | "211-221"xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/pages | "211-221"xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/title | "Identification of blood group A and B antigens in human glycophorin."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/title | "Identification of blood group A and B antigens in human glycophorin."xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/10912628 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10912628 |
| http://purl.uniprot.org/citations/10912628 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10912628 |
| http://purl.uniprot.org/citations/10912628 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10912628 |
| http://purl.uniprot.org/citations/10912628 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10912628 |
| http://purl.uniprot.org/uniprot/P02724 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10912628 |
| http://purl.uniprot.org/uniprot/P02724#attribution-5BF1095B3D6F15123F11C8C262FF451C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10912628 |
| http://purl.uniprot.org/uniprot/#_P02724-citation-10912628 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10912628 |