| http://purl.uniprot.org/citations/11118054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11118054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11118054 | http://www.w3.org/2000/01/rdf-schema#comment | "Thioredoxin (TRX) is a cytoplasmic, redox-sensitive signaling factor believed to participate in the regulation of nuclear transcription factors mediating cellular responses to environmental stress. Activation of the activator protein (AP)-1 transcription factor is thought to be mediated in part by redox-sensitive interactions between the nuclear signaling protein redox factor-1 (Ref-1) and TRX. In this study, the role of TRX and Ref-1 in the activation of the AP-1 complex was examined in HeLa and Jurkat cell lines exposed to ionizing radiation (IR). After exposure to IR, nuclear levels of immunoreactive TRX increased, accompanied by an increase in AP-1 DNA binding activity. It was shown that a physical interaction between Ref-1 and TRX occurs within the nucleus and is enhanced after exposure to IR. Furthermore, TRX immunoprecipitated from irradiated cells was capable of activating AP-1 DNA binding activity in nonirradiated nuclear extracts. In addition, immunodepletion of Ref-1 from nuclear extracts demonstrated that the increase in AP-1 DNA binding activity after IR was also dependent upon the presence of Ref-1 from irradiated cells. Finally, the ability of both TRX and Ref-1 from irradiated cells to stimulate AP-1 DNA binding in nonirradiated nuclear extracts was abolished by chemical oxidation and restored by chemical reduction. These results indicate that, in response to IR, TRX and Ref-1 undergo changes in redox state that contribute to the activation of AP-1 DNA binding activity. These experiments suggest that a redox-sensitive signaling pathway leading from TRX to Ref-1 to the AP-1 complex participates in the up-regulation of DNA binding activity in response to ionizing radiation."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Hirota K."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Hirota K."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Botero A."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Botero A."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Bradbury C.M."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Bradbury C.M."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Gius D."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Gius D."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Goswami P.C."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Goswami P.C."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Laszlo A."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Laszlo A."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Markovina S."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Markovina S."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Spitz D.R."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Spitz D.R."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Wei S.J."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Wei S.J."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Yodoi J."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/author | "Yodoi J."xsd:string |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11118054 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |