| http://purl.uniprot.org/citations/11277933 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11277933 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11277933 | http://www.w3.org/2000/01/rdf-schema#comment | "Human phosphatidylinositol 4-kinase, isoform PI4K92, was expressed as His6 tagged protein in Sf9 cells reaching a level of approximately 5% of cellular protein. The enzyme can be purified nearly to homogeneity in a single step by absorption/desorption on Ni/nitriloacetic acid agarose magnetic beads. High Km values in the millimolar range for ATP and PtdIns as well as only a moderate inhibition by adenosine and a sensitivity to Wortmannin (IC50 approximately 300 nM) characterize the enzyme as a type 3 PI4K. The enzyme produces PtdIns4P as product. The isolated enzyme is a phosphoprotein, additionally phosphate is incorporated by incubation with ATP/Mg or ATP/Mn. Phosphorylation sites were mapped by MALDI-MS and LC-MS/MS at the following positions: S258, T263, S266, S277, S294, T423, S496, T504. Accordingly, a stretch of 81 amino acids between the common and the C-terminal catalytic domain was designated phosphorylation domain."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.02089.x"xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.02089.x"xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Meyer H.E."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Meyer H.E."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Sickmann A."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Sickmann A."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Herberg F.W."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Herberg F.W."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Suer S."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Suer S."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Heilmeyer L.M.G. Jr."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/author | "Heilmeyer L.M.G. Jr."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/pages | "2099-2106"xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/pages | "2099-2106"xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/title | "Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/title | "Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites."xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/volume | "268"xsd:string |
| http://purl.uniprot.org/citations/11277933 | http://purl.uniprot.org/core/volume | "268"xsd:string |