| http://purl.uniprot.org/citations/11278427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11278427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11278427 | http://www.w3.org/2000/01/rdf-schema#comment | "Peflin, a newly identified 30-kDa Ca(2+)-binding protein, belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit, sorcin, grancalcin, and ALG-2 (apoptosis-linked gene 2). We prepared a monoclonal antibody against human peflin. The antibody immunoprecipitated a 22-kDa protein as well as the 30-kDa protein from the lysate of Jurkat cells. Western blotting of the immunoprecipitates revealed that the 22-kDa protein corresponds to ALG-2. This was confirmed by Western blotting of the immunoprecipitates of epitope-tagged peflin or ALG-2 whose cDNA expression constructs were transfected to human embryonic kidney (HEK) 293 cells. Gel filtration of the cytosolic fraction of Jurkat cells revealed co-elution of peflin and ALG-2 in fractions eluting earlier than recombinant ALG-2, further supporting the notion of heterodimerization of the two PEF proteins. Surprisingly, peflin dissociated from ALG-2 in the presence of Ca(2+). Peflin and ALG-2 co-localized in the cytoplasm, but ALG-2 was also detected in the nuclei as revealed by immunofluorescent staining and subcellular fractionation. Peflin was recovered in the cytosolic fraction in the absence of Ca(2+) but in the membrane/cytoskeletal fraction in the presence of Ca(2+). These results suggest that peflin has features common to those of other PEF proteins (dimerization and translocation to membranes) and may modulate the function of ALG-2 in Ca(2+) signaling."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m008649200"xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m008649200"xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Hitomi K."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Hitomi K."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Matsumoto S."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Matsumoto S."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Satoh H."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Satoh H."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Kitaura Y."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/author | "Kitaura Y."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/pages | "14053-14058"xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/pages | "14053-14058"xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/title | "Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/title | "Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11278427 | http://purl.uniprot.org/core/volume | "276"xsd:string |