| http://purl.uniprot.org/citations/11302752 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11302752 | http://www.w3.org/2000/01/rdf-schema#comment | "HL-60 promyelocytic cells were treated with retinoic acid (RA) to stimulate granulocyte differentiation. CCAAT/enhancer binding protein alpha (C/EBPalpha) is known to be the molecular switch during early hematopoietic developmental events that direct cells to the granulocytic pathway. Here we show that the coactivator activating signal cointegrator-2 (ASC-2) plays an important role in differentiation of HL-60 cells into granulocytes by mediating C/EBPalpha-induced gene transcription. The differentiation inducer RA increased mRNA and protein expression of ASC-2. The protein-protein interaction of C/EBPalpha and ASC-2 was detected by coimmunoprecipitation during granulocyte differentiation. Subsequently, GST-pull-down assay revealed that the N-terminal transactivation domain of C/EBPalpha could interact with ASC-2. This functional interaction of ASC-2 with C/EBPalpha drove a synergistic enhancement of C/EBPalpha-dependent transactivation and overexpression of the N-terminal C/EBPalpha protein in HL-60 cells inhibited ASC-2 responsiveness for C/EBPalpha activity in granulocyte differentiation, indicating C/EBPalpha dependency of ASC-2 activity. Taken together, these results suggest that the differentiation-dependent expressed ASC-2 protein physically and functionally interacts with C/EBPalpha and increases its transactivation activity, regulating specific gene transcription for granulocyte differentiation."xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2001.4727"xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/author | "Hong S."xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/author | "Lee M.Y."xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/author | "Cheong J."xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/pages | "1257-1262"xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/title | "Functional interaction of transcriptional coactivator ASC-2 and C/EBPalpha in granulocyte differentiation of HL-60 promyelocytic cell."xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/11302752 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11302752 |
| http://purl.uniprot.org/citations/11302752 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11302752 |
| http://purl.uniprot.org/uniprot/Q14686#attribution-E5EB0CDC35C722D0DFFAF974DA6095A4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11302752 |