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http://purl.uniprot.org/citations/11340635http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11340635http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11340635http://www.w3.org/2000/01/rdf-schema#comment"

Background

The database of human Expressed Sequence Tags (dbEST) provides a potential source for identification of tissue-specific genes. This database contains sequences that originate from cDNA libraries from particular tumors, organs or cell types. In this report, we have used the EST database to identify PRAC, a novel gene specifically expressed in human Prostate, prostate cancer, Rectum And distal Colon.

Methods

Using a computer based analysis, a cluster of sequence homologous ESTs was identified which contained ESTs derived only from human prostate cDNA libraries. The tissue specificity was examined by multiple tissue RNA dot blots and RT-PCR. The PRAC transcript and protein was identified using Northern blot analysis, RACE-PCR, primer extension, and western blot.

Results

PRAC encode a 382 nucleotide RNA found in prostate, rectum, distal colon, and in three prostate cancer cell lines; LNCaP, PC-3 and DU145. This transcript encodes a 6 kDa nuclear protein. The PRAC gene is located on chromosome 17 at position 17q21, about 4 kbp downstream from the homeodomain Hoxb-13 gene.

Conclusions

Our data proves that the EST database can be a useful tool for discovery of prostate-specific genes. The nuclear localization, identification of potential phosphorylation sites, and possible cotranscription with the Hoxb-13 gene suggest that PRAC may have a regulatory role in the nucleus."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.org/dc/terms/identifier"doi:10.1002/pros.1055"xsd:string
http://purl.uniprot.org/citations/11340635http://purl.org/dc/terms/identifier"doi:10.1002/pros.1055"xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Lee B."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Lee B."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Liu X.F."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Liu X.F."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Bera T.K."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Bera T.K."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Pastan I."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Pastan I."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Wolfgang C.D."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Wolfgang C.D."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Olsson P."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Olsson P."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Duray P."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/author"Duray P."xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/name"Prostate"xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/name"Prostate"xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/pages"125-131"xsd:string
http://purl.uniprot.org/citations/11340635http://purl.uniprot.org/core/pages"125-131"xsd:string
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