| http://purl.uniprot.org/citations/11342538 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11342538 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11342538 | http://www.w3.org/2000/01/rdf-schema#comment | "The ubiquitin-protein ligase (E3), hRPF1/Nedd4, is a component of the ubiquitin-proteasome pathway responsible for substrate recognition and specificity. Although previously characterized as a regulator of the stability of cytoplasmic proteins, hRPF1/Nedd4 has also been suggested to have a role in the nucleus. However, in light of the cytoplasmic localization of hRPF1/Nedd4, it is unclear whether bona fide nuclear substrates of hRPF1/Nedd4 exist, and if so, what mechanism may allow a cytoplasmic ubiquitin ligase to manifest nuclear activity. Our search for nuclear substrates led to the identification of the human proline-rich transcript, brain-expressed (hPRTB) protein, the ubiquitination and degradation of which is regulated by hRPF1/Nedd4. Interestingly, hPRTB colocalizes with the splicing factor SC35 in nuclear speckles. Finally, we demonstrate that hRPF1/Nedd4 is indeed capable of entering the nucleus; however, the presence of a functional Rev-like nuclear export sequence in hRPF1/Nedd4 ensures a predominant cytoplasmic localization. Cumulatively, these findings highlight a nuclear role for the ubiquitin ligase hRPF1/Nedd4 and underscore cytoplasmic/nuclear localization as an important regulatory component of hRPF1/Nedd4-substrate recognition."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101205200"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101205200"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Huibregtse J.M."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Huibregtse J.M."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Tcherepanova I."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Tcherepanova I."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "McDonnell D.P."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "McDonnell D.P."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Hamilton M.H."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/author | "Hamilton M.H."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/pages | "26324-26331"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/pages | "26324-26331"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/title | "Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/title | "Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates."xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11342538 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11342538 |
| http://purl.uniprot.org/citations/11342538 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11342538 |