| http://purl.uniprot.org/citations/11425856 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11425856 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11425856 | http://www.w3.org/2000/01/rdf-schema#comment | "The first extracellular domain (ECD-1) of the corticotropin releasing factor (CRF) type 1 receptor, (CRFR1), is important for binding of CRF ligands. A soluble protein, mNT-CRFR1, produced by COS M6 cells transfected with a cDNA encoding amino acids 1--119 of human CRFR1 and modified to include epitope tags, binds a CRF antagonist, astressin, in a radioreceptor assay using [(125)I-d-Tyr(0)]astressin. N-terminal sequencing of mNT-CRFR1 showed the absence of the first 23 amino acids of human CRFR1. This result suggests that the CRFR1 protein is processed to cleave a putative signal peptide corresponding to amino acids 1--23. A cDNA encoding amino acids 24--119 followed by a FLAG tag, was expressed as a thioredoxin fusion protein in Escherichia coli. Following thrombin cleavage, the purified protein (bNT-CRFR1) binds astressin and the agonist urocortin with high affinity. Reduced, alkylated bNT-CRFR1 does not bind [(125)I-D-Tyr(0)]astressin. Mass spectrometric analysis of photoaffinity labeled bNT-CRFR1 yielded a 1:1 complex with ligand. Analysis of the disulfide arrangement of bNT-CRFR1 revealed bonds between Cys(30) and Cys(54), Cys(44) and Cys(87), and Cys(68) and Cys(102). This arrangement is similar to that of the ECD-1 of the parathyroid hormone receptor (PTHR), suggesting a conserved structural motif in the N-terminal domain of this family of receptors."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101838200"xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101838200"xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Fischer W.H."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Fischer W.H."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Koerber S.C."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Koerber S.C."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Vale W.W."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Vale W.W."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Greenwald J."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Greenwald J."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Craig A.G."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Craig A.G."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Rivier J.E."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Rivier J.E."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Cervini L.A."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Cervini L.A."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Groppe J.C."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Groppe J.C."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Kunitake K.S."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Kunitake K.S."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Moller Nielsen S."xsd:string |
| http://purl.uniprot.org/citations/11425856 | http://purl.uniprot.org/core/author | "Moller Nielsen S."xsd:string |