| http://purl.uniprot.org/citations/11533048 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11533048 | http://www.w3.org/2000/01/rdf-schema#comment | "S-Nitrosoglutathione and the dinitrosyl-diglutathionyl iron complex are involved in the storage and transport of NO in biological systems. Their interactions with the human glutathione transferase P1-1 may reveal an additional physiological role for this enzyme. In the absence of GSH, S-nitrosoglutathione causes rapid and stable S-nitrosylation of both the Cys(47) and Cys(101) residues. Ion spray ionization-mass spectrometry ruled out the possibility of S-glutathionylation and confirms the occurrence of a poly-S-nitrosylation in GST P1-1. S-Nitrosylation of Cys(47) lowers the affinity 10-fold for GSH, but this negative effect is minimized by a half-site reactivity mechanism that protects one Cys(47)/dimer from nitrosylation. Thus, glutathione transferase P1-1, retaining most of its original activity, may act as a NO carrier protein when GSH depletion occurs in the cell. The dinitrosyl-diglutathionyl iron complex, which is formed by S-nitrosoglutathione decomposition in the presence of physiological concentrations of GSH and traces of ferrous ions, binds with extraordinary affinity to one active site of this dimeric enzyme (K(i) < 10(-12) m) and triggers negative cooperativity in the vacant subunit (K(i) = 10(-9) m). The complex bound to the enzyme is stable for hours, whereas in the free form and at low concentrations, its life time is only a few minutes. ESR and molecular modeling studies provide a reasonable explanation of this strong interaction, suggesting that Tyr(7) and enzyme-bound GSH could be involved in the coordination of the iron atom. All of the observed findings suggest that glutathione transferase P1-1, by means of an intersubunit communication, may act as a NO carrier under different cellular conditions while maintaining its well known detoxificating activity toward dangerous compounds."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m102344200"xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Parker M.W."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Rossjohn J."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "McKinstry W.J."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Caccuri A.M."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Federici G."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Lo Bello M."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Ricci G."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Stella L."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Pedersen J.Z."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Polizio F."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Nuccetelli M."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/author | "Mozzi A.F."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/pages | "42138-42145"xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/title | "Human glutathione transferase P1-1 and nitric oxide carriers; a new role for an old enzyme."xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11533048 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11533048 |
| http://purl.uniprot.org/citations/11533048 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11533048 |
| http://purl.uniprot.org/uniprot/P09211#attribution-54F7665D60173B18088BF228C6DA0D63 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11533048 |
| http://purl.uniprot.org/uniprot/P09211#attribution-CBC8523AA73500765CDA87258ADB9462 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11533048 |