| http://purl.uniprot.org/citations/11559703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11559703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11559703 | http://www.w3.org/2000/01/rdf-schema#comment | "In Caenorhabditis elegans, fem-1, fem-2, and fem-3 play pivotal roles in sex determination. Recently, a mammalian homologue of the C. elegans sex-determining protein FEM-1, F1Aalpha, has been described. Although there is little evidence to link F1Aalpha to sex determination, F1Aalpha and FEM-1 both promote apoptosis in mammalian cells. Here we report the identification and characterization of a human homologue of the C. elegans sex-determining protein FEM-2, hFEM-2. Similar to FEM-2, hFEM-2 exhibited PP2C phosphatase activity and associated with FEM-3. hFEM-2 shows striking similarity (79% amino acid identity) to rat Ca(2+)/calmodulin (CaM)-dependent protein kinase phosphatase (rCaMKPase). hFEM-2 and FEM-2, but not PP2Calpha, were demonstrated to dephosphorylate CaM kinase II efficiently in vitro, suggesting that hFEM-2 and FEM-2 are specific phosphatases for CaM kinase. Furthermore, hFEM-2 and FEM-2 associated with F1Aalpha and FEM-1 respectively. Overexpression of hFEM-2, FEM-2, or rCaMKPase all mediated apoptosis in mammalian cells. The catalytically active, but not the inactive, forms of hFEM-2 induced caspase-dependent apoptosis, which was blocked by Bcl-XL or a dominant negative mutant of caspase-9. Taken together, our data suggest that hFEM-2 and rCaMKPase are mammalian homologues of FEM-2 and they are evolutionarily conserved CaM kinase phosphatases that may have a role in apoptosis signaling."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m105880200"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m105880200"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Chan S.-L."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Chan S.-L."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Tan K.M.L."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Tan K.M.L."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Tan K.O."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Tan K.O."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Yu V.C."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/author | "Yu V.C."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/pages | "44193-44202"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/pages | "44193-44202"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/title | "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/title | "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11559703 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11559703 |
| http://purl.uniprot.org/citations/11559703 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11559703 |