http://purl.uniprot.org/citations/11588157 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11588157 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11588157 | http://www.w3.org/2000/01/rdf-schema#comment | "Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked oligosaccharides if beta-1,4-galactosyltransferase (beta-1,4-GalT) is supplied. The full-length human beta-1,4-GalT I, II, III, IV, V, and VI cDNAs were independently transfected into Sf-9 cells, and the galactosylation of endogenous membrane glycoproteins was examined by lectin blot analysis using Ricinus communis agglutinin-I (RCA-I), which preferentially interacts with oligosaccharides terminated with Galbeta1-->4GlcNAc group. Several RCA-I-reactive bands appeared in all of the gene-transfected cells, and disappeared on treatment of blots with beta-1,4-galactosidase or N-glycanase prior to incubation with lectin. Introduction of the antisense beta-1,4-GalT II and V cDNAs separately into human colorectal adenocarcinoma SW480 cells, in which beta-1,4-GalT I, II, and V genes were expressed, resulted in the reduction of RCA-I binding toward N-linked oligosaccharides of the membrane glycoproteins. Differences were found in their K(m) values toward UDP-Gal and GlcNAcbeta-S-pNP and in their acceptor specificities toward oligosaccharides with the GlcNAcbeta1-->4(GlcNAcbeta1-->2)Man branch and with the GlcNAcbeta1-->6(GlcNAcbeta1-->2)Man branch. These results indicate that beta-1,4-GalTs II, III, IV, V, and VI are involved in the N-linked oligosaccharide biosynthesis cooperatively but not in a redundant manner with beta-1,4-GalT I within cells."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/11.10.813"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/11.10.813"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Guo S."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Guo S."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Furukawa K."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Furukawa K."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Shirane K."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/author | "Shirane K."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/pages | "813-820"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/pages | "813-820"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/title | "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/title | "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/volume | "11"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://purl.uniprot.org/core/volume | "11"xsd:string |
http://purl.uniprot.org/citations/11588157 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11588157 |
http://purl.uniprot.org/citations/11588157 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11588157 |