| http://purl.uniprot.org/citations/11606566 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11606566 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11606566 | http://www.w3.org/2000/01/rdf-schema#comment | "Phosphoinositide 3-kinase C2alpha (PI3K-C2alpha) belongs to the class II phosphatidylinositol 3-kinases, which are defined by their in vitro usage of phosphatidylinositol and phosphatidylinositol 4-phosphate as substrates. All type II phosphatidylinositol 3-kinases contain at their C terminus a C2-like domain. Here we demonstrate that Homo sapiens phosphoinositide 3-kinase C2alpha (HsPI3K-C2alpha) has dual cellular localization present in the cytoplasm and in the nucleus. A distinct nuclear localization signal sequence was identified by expressing HsPI3K-C2alpha-green fluorescent protein fusion proteins in HeLa cells. The nuclear localization signal was mapped to a stretch of 11 amino acids (KRKTKISRKTR) located within C2-like domain of the kinase. In the cytoplasm and the nucleus HsPI3K-C2alpha associates with macromolecular complexes that are resistant to detergent extraction. Indirect immunofluorescence reveals that in the nucleus HsPI3K-C2alpha is enriched at distinct subnuclear domains known as nuclear speckles, which contain pre-mRNA processing factors and are functionally connected to RNA metabolism. Phosphorylation of HsPI3K-C2alpha is induced by inhibition of RNA polymerase II-dependent transcription and coincides with enlargement and rounding up of the nuclear speckles. The results suggest that phosphorylation of HsPI3K-C2alpha is inversely linked to mRNA transcription and supports the importance of phosphoinositides for nuclear activity."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104610200"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104610200"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/author | "Thelen M."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/author | "Thelen M."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/author | "Didichenko S.A."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/author | "Didichenko S.A."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/pages | "48135-48142"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/pages | "48135-48142"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/title | "Phosphatidylinositol 3-kinase c2alpha contains a nuclear localization sequence and associates with nuclear speckles."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/title | "Phosphatidylinositol 3-kinase c2alpha contains a nuclear localization sequence and associates with nuclear speckles."xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11606566 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11606566 |
| http://purl.uniprot.org/citations/11606566 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11606566 |
| http://purl.uniprot.org/citations/11606566 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11606566 |
| http://purl.uniprot.org/citations/11606566 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11606566 |
| http://purl.uniprot.org/uniprot/O00443 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11606566 |
| http://purl.uniprot.org/uniprot/O00443#attribution-56CD57A0755511CDED260724E1AAB39D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11606566 |