| http://purl.uniprot.org/citations/11883939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11883939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11883939 | http://www.w3.org/2000/01/rdf-schema#comment | "The apoptosis-linked protein ALG-2 is a Ca(2+)-binding protein that belongs to the penta-EF-hand protein family. ALG-2 forms a homodimer, a heterodimer with another penta-EF-hand protein, peflin, and a complex with its interacting protein, named AIP1 or Alix. By yeast two-hybrid screening using human ALG-2 as bait, we isolated a cDNA of a novel ALG-2-interacting protein, which turned out to be annexin XI. Deletion analysis revealed that ALG-2 interacted with the N-terminal domain of annexin XI (AnxN), which has an amino acid sequence similar to that of the C-terminal region of AIP1/Alix. Using recombinant biotin-tagged ALG-2 and the glutathione S-transferase (GST) fusion protein of AnxN, the direct interaction was analyzed by an ALG-2 overlay assay and by real-time interaction analysis with a surface plasmon resonance (SPR) biosensor. The dissociation constant (K(d)) was estimated to be approximately 70 nM. The Ca(2+)-dependent fluorescence change of ALG-2 in the presence of the hydrophobicity fluorescent probe 2-p-toluidinylnaphthalene-6-sulfonate (TNS) was inhibited by mixing with GST-AnxN, suggesting that the Pro/Gly/Tyr/Ala-rich hydrophobic region in AnxN masked the Ca(2+)-dependently exposed hydrophobic surface of ALG-2."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2002.6600"xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2002.6600"xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Nakano Y."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Nakano Y."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Shibata H."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Shibata H."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Satoh H."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Satoh H."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Kitaura Y."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/author | "Kitaura Y."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/pages | "1166-1172"xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/pages | "1166-1172"xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/title | "ALG-2 interacts with the amino-terminal domain of annexin XI in a Ca(2+)-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/title | "ALG-2 interacts with the amino-terminal domain of annexin XI in a Ca(2+)-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/11883939 | http://purl.uniprot.org/core/volume | "291"xsd:string |