| http://purl.uniprot.org/citations/11886864 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11886864 | http://www.w3.org/2000/01/rdf-schema#comment | "The essential Saccharomyces cerevisiae PRP43 gene encodes a 767-amino acid protein of the DEXH-box family. Prp43 has been implicated in spliceosome disassembly (Arenas, J. E., and Abelson, J. N. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 11798-11802). Here we show that purified recombinant Prp43 is an RNA-dependent ATPase. Alanine mutations at conserved residues within motifs I ((119)GSGKT(123)), II ((215)DEAH(218)) and VI ((423)QRAGRAGR(430)) that diminished ATPase activity in vitro were lethal in vivo, indicating that ATP hydrolysis is necessary for the biological function of Prp43. Overexpression of lethal, ATPase-defective mutants in a wild-type strain resulted in dominant-negative growth inhibition. The ATPase-defective mutant T123A interfered in trans with the in vitro splicing function of wild-type Prp43. T123A did not affect the chemical steps of splicing or the release of mature mRNA from the spliceosome, but it blocked the release of the excised lariat-intron from the spliceosome. We show that the lariat-intron is not accessible to debranching by purified Dbr1 when it is held in the T123A-arrested splicing complex. Our results define a new ATP-dependent step of splicing that is catalyzed by Prp43."xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200762200"xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/author | "Martin A."xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/author | "Schneider S."xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/author | "Schwer B."xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/pages | "17743-17750"xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/title | "Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome."xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11886864 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11886864 |
| http://purl.uniprot.org/citations/11886864 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11886864 |
| http://purl.uniprot.org/uniprot/P53131#attribution-1191AC2BED7BAB08CC3FC75C58BE7147 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11886864 |
| http://purl.uniprot.org/uniprot/#_P53131-mappedCitation-11886864 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11886864 |
| http://purl.uniprot.org/uniprot/P53131 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11886864 |