| http://purl.uniprot.org/citations/11935223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11935223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11935223 | http://www.w3.org/2000/01/rdf-schema#comment | "The essential Saccharomyces cerevisiae gene YLR186w [ NEP1 (nucleolar essential protein 1), also recently named EMG1 (essential for mitotic growth 1)] is highly conserved in eukaryotes and archaea. In S. cerevisiae, Nep1p is localized in the nucleus with a rod-shaped morphology, which first suggested a spindle/microtubule association for ScNEP1 (S. cerevisiae NEP1). However, in Candida albicans and in HeLa cells, Nep1p is a protein of the nucleoli. CaNEP1 (C. albicans NEP1) and HsNEP1 (Homo sapiens NEP1) heterologously complement the essential phenotype in a S. cerevisiae nep1 deletion mutant. However, the ScNEP1 spindle/microtubule phenotype is not found with HsNEP1 and CaNEP1, which shows that the spindle/microtubule association is specific for ScNep1p and that it is not the primary essential function of Nep1p. A temperature-sensitive ScNEP1 (ts1) allele was isolated and revealed a strongly increased sensitivity to paromomycin, a translational inhibitor which binds to RNA, indicating that ribosome biogenesis within the nucleolus is probably affected. This was confirmed by polysome profile and ribosomal subunit analysis, showing a ribosomal subunit imbalance with a decrease in 40S subunits, due to reduced amounts of native 18S rRNA. Furthermore, ScSAM2, encoding S-adenosylmethionine synthetase, was isolated as a multicopy-suppressor of the ScNEP1 (ts1) mutant allele and medium supplementation with S-adenosylmethionine restored its growth. For the first time, these results identified a novel protein with an essential function in ribosomal biogenesis which directly or indirectly interferes with a methylation reaction during the early steps of pre-rRNA processing necessary for the generation of 40S ribosomal subunits."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00294-001-0269-4"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00294-001-0269-4"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Entian K.-D."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Entian K.-D."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Koetter P."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Koetter P."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Eschrich D."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Eschrich D."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Buchhaupt M."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/author | "Buchhaupt M."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/name | "Curr. Genet."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/name | "Curr. Genet."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/pages | "326-338"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/pages | "326-338"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/title | "Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/title | "Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis."xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/11935223 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11935223 |
| http://purl.uniprot.org/citations/11935223 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11935223 |