| http://purl.uniprot.org/citations/12130530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12130530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12130530 | http://www.w3.org/2000/01/rdf-schema#comment | "Secretion of platelet granules is necessary for normal hemostasis. Platelet secretion requires soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complex formation between different members of the syntaxin, SNAP-25, and vesicle-associated membrane protein (VAMP) gene families. Using microcapillary reverse-phase high-performance liquid chromatography-nano-electrospray tandem mass spectrometry, we identified VAMP-3 and VAMP-8 as VAMP isoforms coimmunoprecipitated from platelets with syntaxin 4. Immunoblotting experiments confirmed the presence of VAMP-3 and VAMP-8 but not VAMP-1 or VAMP-2 in platelets. To examine the effect of VAMP proteins on platelet secretion, soluble recombinant (r) VAMP-2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets. Secretion of alpha granules (monitored by flow cytometric measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 14-serotonin) was almost completely inhibited by rVAMP-3, whereas rVAMP-8 inhibited secretion of dense granules but not alpha granules. In contrast, rVAMP-2, which formed SNARE complexes in vitro, had no effect on platelet exocytosis. We conclude that VAMP-3 and VAMP-8 form SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood.v100.3.1081"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood.v100.3.1081"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Chung S.H."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Chung S.H."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Polgar J."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Polgar J."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Reed G.L."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/author | "Reed G.L."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/pages | "1081-1083"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/pages | "1081-1083"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/title | "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/title | "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion."xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12130530 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12130530 |
| http://purl.uniprot.org/citations/12130530 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12130530 |
| http://purl.uniprot.org/citations/12130530 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12130530 |
| http://purl.uniprot.org/citations/12130530 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12130530 |