| http://purl.uniprot.org/citations/12372837 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12372837 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12372837 | http://www.w3.org/2000/01/rdf-schema#comment | "The identification of phosphoinositide-dependent kinase-1 (PDK-1) as an activating kinase for members of the AGC family of kinases has led to its implication as the activating kinase for cAMP-dependent protein kinase. It has been established in vitro that PDK-1 can phosphorylate the catalytic (C) subunit (), but the Escherichia coli-expressed C-subunit undergoes autophosphorylation. To assess which of these mechanisms occurs in mammalian cells, a set of mutations was engineered flanking the site of PDK-1 phosphorylation, Thr-197, on the activation segment of the C-subunit. Two distinct requirements appeared for autophosphorylation and phosphorylation by PDK-1. Autophosphorylation was disrupted by mutations that compromised activity (Thr-201 and Gly-200) or altered substrate recognition (Arg-194). Conversely, only residues peripheral to Thr-197 altered PDK-1 phosphorylation, including a potential hydrophobic PDK-1 binding site at the C terminus. To address the in vivo requirements for phosphorylation, select mutant proteins were transfected into COS-7 cells, and their phosphorylation state was assessed with phospho-specific antibodies. The phosphorylation pattern of these mutant proteins indicates that autophosphorylation is not the maturation mechanism in the eukaryotic cell; instead, a heterologous kinase with properties resembling the in vitro characteristics of PDK-1 is responsible for in vivo phosphorylation of PKA."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204970200"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204970200"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Taylor S.S."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Taylor S.S."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Moore M.J."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Moore M.J."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Jones K.C."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Jones K.C."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Kanter J.R."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/author | "Kanter J.R."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/pages | "47878-47884"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/pages | "47878-47884"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/title | "Phosphorylation of the catalytic subunit of protein kinase A. Autophosphorylation versus phosphorylation by phosphoinositide-dependent kinase-1."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/title | "Phosphorylation of the catalytic subunit of protein kinase A. Autophosphorylation versus phosphorylation by phosphoinositide-dependent kinase-1."xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/12372837 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12372837 |
| http://purl.uniprot.org/citations/12372837 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12372837 |